Experiment Overview
| Repository ID: | FR-FCM-ZZCB | Experiment name: | Effect of monensin on IFNg mRNA and Protein expression | MIFlowCyt score: | 100.00% |
| Primary researcher: | Chip Lomas | PI/manager: | Emily Park | Uploaded by: | Chip Lomas |
| Experiment dates: | 2013-12-17 - 2013-12-18 | Dataset uploaded: | May 2014 | Last updated: | Aug 2014 |
| Keywords: | [PBMC] [Gene expression] [RNA Flow cytometry] | Manuscripts: | [25123411] |
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| Organizations: |
BD Biosciences, Research and Development, San Jose, CA (USA)
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| Purpose: | The purpose of the experiment was to determine the effect of the addition of monensin on IFNg mRNA and protein expression in PMA/Ionomycin stimulated PBMCs. | ||||
| Conclusion: | When monensin is included, there is a mRNA+/protein- population observed after 5 hour stimulation. This population is not observed when monensin is omitted. | ||||
| Comments: | Figure 4A-B: Simultaneous flow cytometric analysis of IFN-g and CD4 mRNA and protein expression kinetics in human peripheral blood mononuclear cells during activation | ||||
| Funding: | Not disclosed | ||||
| Quality control: | Instrument was set up with CST in FACSDiva software. | ||||
Experiment variables
| Timepoints | |
|---|---|
| · 0 min | RNA_T0-PMA Iono monensin_009.fcs · RNA_T0-PMA Iono_005.fcs · RNA_T0-no stim_001.fcs |
| · 60 min | RNA_T60-PMA Iono monensin_010.fcs · RNA_T60-PMA Iono_006.fcs · RNA_T60-no stim_002.fcs |
| · 300 min | RNA_T5-PMA Iono monensin_011.fcs · RNA_T5-PMA Iono monensini-NTC_012.fcs · RNA_T5-no stim-NTC_004.fcs · RNA_T5-no stim_003.fcs · RNA_T5-PMA Iono-NTC_008.fcs · RNA_T5-PMA Iono_007.fcs |
| Sample Type | |
|---|---|
| · Experimental Sample | RNA_T0-PMA Iono monensin_009.fcs · RNA_T0-PMA Iono_005.fcs · RNA_T0-no stim_001.fcs · RNA_T5-PMA Iono monensin_011.fcs · RNA_T5-no stim_003.fcs · RNA_T5-PMA Iono_007.fcs · RNA_T60-PMA Iono monensin_010.fcs · RNA_T60-PMA Iono_006.fcs · RNA_T60-no stim_002.fcs |
| · No Target Probe added-negative sample | RNA_T5-PMA Iono monensini-NTC_012.fcs · RNA_T5-no stim-NTC_004.fcs · RNA_T5-PMA Iono-NTC_008.fcs |
| Conditions | |
|---|---|
| · Rested | RNA_T0-no stim_001.fcs · RNA_T5-no stim-NTC_004.fcs · RNA_T5-no stim_003.fcs · RNA_T60-no stim_002.fcs |
| · PMA/Ionomycin | RNA_T0-PMA Iono_005.fcs · RNA_T5-PMA Iono-NTC_008.fcs · RNA_T5-PMA Iono_007.fcs · RNA_T60-PMA Iono_006.fcs |
| · PMA/Ionomycin + Monensin | RNA_T0-PMA Iono monensin_009.fcs · RNA_T5-PMA Iono monensin_011.fcs · RNA_T5-PMA Iono monensini-NTC_012.fcs · RNA_T60-PMA Iono monensin_010.fcs |
