Experiment Overview
Repository ID: | FR-FCM-ZZCC | Experiment name: | RNA Flow Cytometry: K562 BCR mRNA expression. Sorting for imaging correlation | MIFlowCyt score: | 97.75% |
Primary researcher: | Chip Lomas | PI/manager: | Emily Park | Uploaded by: | Chip Lomas |
Experiment dates: | 2012-07-10 - 2012-07-10 | Dataset uploaded: | May 2014 | Last updated: | May 2014 |
Keywords: | [Gene expression] [RNA Amplification] [RNA hybridization] [RNA Flow cytometry] | Manuscripts: | [23441230] [PMC3575505] | ||
Organizations: |
BD Biosciences, Research and Development, San Jose, CA (USA)
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Purpose: | K562 cells were sorted based on MFI onto slides for imaging correlation of BCR mRNA expression. | ||||
Conclusion: | BCR mRNA expression was well correlated between MFI (flow) and spot count (imaging) for all gates (High, mid, low, negative). | ||||
Comments: | Detection of Low Abundance RNA Molecules in Individual Cells by Flow Cytometry Figure 5e | ||||
Funding: | Not disclosed | ||||
Quality control: | Instrument was setup with CST in FACSDiva software. |
Experiment variables
Sample Type | |
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· No Target Probe Negative Control | 90% k562 10% PBMC Mixture_No Target Probe Control_001.fcs |
· Stained Sample | 90% k562 10% PBMC Mixture_Stained Sample_002.fcs |