Experiment Overview
| Repository ID: | FR-FCM-ZZCE | Experiment name: | RNA Flow Cytometry: multiplexing evaluation | MIFlowCyt score: | 97.75% |
| Primary researcher: | Chip Lomas | PI/manager: | Emily Park | Uploaded by: | Chip Lomas |
| Experiment dates: | 2012-05-01 - 2012-05-01 | Dataset uploaded: | May 2014 | Last updated: | May 2014 |
| Keywords: | [Gene expression] [RNA Amplification] [RNA hybridization] [RNA Flow cytometry] | Manuscripts: | [23441230] [PMC3575505] |
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| Organizations: |
BD Biosciences, Research and Development, San Jose, CA (USA)
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| Purpose: | The purpose was to evaluate the multiplexing capability of RNA flow cytometry by staining 3 mRNA targets simultaneously. | ||||
| Conclusion: | After RNA staining, we were able to confirm positive staining for all three targets: BCR, ABL, and 18s. Cells were sorted and also validated by imaging. | ||||
| Comments: | Detection of Low Abundance RNA Molecules in Individual Cells by Flow Cytometry Figure 4A-B | ||||
| Funding: | Not disclosed | ||||
| Quality control: | Instrument was setup using CST in FACSDiva software. | ||||
Experiment variables
| Sample Type | |
|---|---|
| · No Target Probe Negative Control | k562_NTC_002.fcs |
| · Stained Sample | k562_BCR_ABL_18s_001.fcs |
