Experiment Overview

Repository ID: FR-FCM-ZZCF Experiment name: RNA Flow Cytometry: evaluation of detection sensitivity in low abundant intracellular RNA MIFlowCyt score: 97.75%
Primary researcher: Chip Lomas PI/manager: Emily Park Uploaded by: Chip Lomas
Experiment dates: 2012-04-25 - 2012-04-25 Dataset uploaded: May 2014 Last updated: May 2014
Keywords: [Gene expression] [RNA Amplification] [RNA hybridization] [RNA Flow cytometry] Manuscripts: [23441230] [PMC3575505] Plosonelogo
Organizations: BD Biosciences, Research and Development, San Jose, CA (USA)
Purpose: The purpose of the experiment was to determine sensitivity of RNA flow cytometry by analyzing samples with low abundant intracellular RNA. Slides made from the suspension stained samples were run by flow and were also imaged on slides. MFI from the flow analysis was correlated to RNA spot count from the imaging analysis to determine sensitivity.
Conclusion: It was determined from this experiment that it is possible to distinguish cells containing only 1-2 spots by flow cytometry, by correlating MFI values of gated cells to the spot counts generated by image analysis.
Comments: Detection of Low Abundance RNA Molecules in Individual Cells by Flow Cytometry Figures 5A-D
Funding: Not disclosed
Quality control: Instrument was setup with CST in FACSDiva software.

Experiment variables

Sample Type
· No Target Probe Negative Control K562 Cells_No Target Probes_002.fcs
· Stained Sample K562 Cells_BCR-A647_001.fcs

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