Experiment Overview
| Repository ID: | FR-FCM-ZZDK | Experiment name: | Lina Han-Flow-CyTOF | MIFlowCyt score: | 27.00% |
| Primary researcher: | LINA HAN | PI/manager: | LINA HAN | Uploaded by: | LINA HAN |
| Experiment dates: | 2012-10-31 - 2013-07-24 | Dataset uploaded: | Jun 2014 | Last updated: | Jan 2015 |
| Keywords: | [Acute Myeloid Leukemia] [multicolor flow cytometry] [signaling] | Manuscripts: |
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| Organizations: | None | ||||
| Purpose: | To validate CyTOF data using flow cytometry | ||||
| Conclusion: | Frequencies of LSC phenotypes and cytokine-induced intracellular signals are consistent between flow cytometry and CyTOF | ||||
| Comments: | None | ||||
| Funding: | This work was supported by the Leukemia and Lymphoma Society grant 6427-13 (To M.G. and M.K.). National Institutes of Health/National Cancer Institute grants P01 CA55164, P30 CA016672, P50 CA100632, R01 CA163481, a Cancer Prevention and Research Institute of Texas Shared Instrument Award, and the Paul and Mary Haas Chair in Genetics (to M.A.). | ||||
| Quality control: | To standardize voltage settings across samples acquired on different days, single stained controls were included. Voltages were adjusted such that fluorescence intensity was identicla for each antibody. | ||||
