Experiment Overview
| Repository ID: | FR-FCM-ZZEK | Experiment name: | P. putida KT2440 cell sorting by DNA content | MIFlowCyt score: | 79.50% |
| Primary researcher: | Michael Jahn | PI/manager: | Michael Jahn | Uploaded by: | Michael Jahn |
| Experiment dates: | 2012-12-04 - 2012-12-04 | Dataset uploaded: | Aug 2014 | Last updated: | Oct 2014 |
| Keywords: | [Pseudomonas putida] [DNA content] [DAPI] | Manuscripts: | |||
| Organizations: |
Helmholtz-Centre for Environmental Research - UFZ, Env. microbiology, Leipzig, (Germany)
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| Purpose: | To identify differences between sub-populations of different DNA content in Pseudomonas putida KT2440, cultivated in chemostat at different growth rates. Sub-populations were sorted (5x10^6 cells) and analyzed by protein mass spectrometry. | ||||
| Conclusion: | Only slight differences were found between DNA sub-populations of identical growth rate, when cells were sorted and analyzed by LC-MS/MS. However, strong differences in proteome profile were detected between cells of different growth rate. | ||||
| Comments: | Published as: Lieder S, Jahn M, Seifert J,von Bergen M, Müller S, Takors R. Subpopulation-proteomics reveal growth rate, but not cell cycling, as a major impact on protein composition in Pseudomonas putida KT2440. AMB Express 2014, 4:71. http://www.amb-express.com/content/4/1/71 | ||||
| Funding: | ERA-IB (European Union), Sächsische Aufbaubank (Saxony, Germany) | ||||
| Quality control: | Two biological replicates. Calibration of instrument with fluorescent beads and biological standard (DAPI stained) | ||||
Experiment variables
| Conditions | |
|---|---|
| · Bead control | AMJ_04_Dec_12_03.fcs · AMJ_04_Dec_12_04.fcs |
| · D=0.1 | AMJ_04_Dec_12_06.fcs · AMJ_04_Dec_12_07.fcs |
| · D=0.2 | AMJ_04_Dec_12_08.fcs · AMJ_04_Dec_12_09.fcs |
| · D=0.7 | AMJ_04_Dec_12_10.fcs · AMJ_04_Dec_12_11.fcs |
| · Biological standard | AMJ_04_Dec_12_05.fcs |
