Experiment Overview
| Repository ID: | FR-FCM-ZZEL | Experiment name: | BODIPY® FL Histamine as a new modality for quantitative detection of histamine receptor upregulation upon IgE sensitization in murine bone marrow-derived mast cells | MIFlowCyt score: | 83.75% |
| Primary researcher: | Arash Mirzahosseini | PI/manager: | Arash Mirzahosseini | Uploaded by: | Arash Mirzahosseini |
| Experiment dates: | 2012-04-04 - 2012-07-03 | Dataset uploaded: | Aug 2014 | Last updated: | Sep 2014 |
| Keywords: | [histamine H4 receptor] [BODIPY® FL Histamine] [mast cells] | Manuscripts: |
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| Organizations: |
Sanofi Research and Development, Budapest, Immuno-Inflammation Unit, Budapest, Select a State (Magyarország)
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| Purpose: | The purpose of the experiments presented was to test whether histamine receptors can be analyzed by staining with BODIPY® FL Histamine, a fluorescent ligand, using flow cytometry. | ||||
| Conclusion: | BODIPY® FL Histamine was found to stain H4 transfected cells and mast cells in a concentration-dependent manner; this staining was competitively inhibited with unlabeled ligands. Furthermore, IgE sensitization was found to increase H4 receptor expression in mast cells. | ||||
| Comments: | None | ||||
| Funding: | This work was supported by Sanofi (Budapest, Hungary). | ||||
| Quality control: | The cell phenotype (CF117, FcεRI) and immunocytostaining controls are described in the Materials and Methods. | ||||
Experiment variables
| Doses | |
|---|---|
| · 1e-4 | Parental_1e-4.fcs · Transf_1e-4.fcs |
| · 1e-5 | Parental_1e-5.fcs · Transf_1e-5.fcs |
| · 1e-6 | Parental_1e-6.fcs · Transf_1e-6.fcs |
| · 1e-7 | Parental_1e-7.fcs · Transf_1e-7.fcs |
| · 1e-8 | Parental_1e-8.fcs · Transf_1e-8.fcs |
| · 1e-9 | Parental_1e-9.fcs · Transf_1e-9.fcs |
