Experiment Overview
| Repository ID: | FR-FCM-ZZG8 | Experiment name: | Exploration of innate immune cells populations in PBMC | MIFlowCyt score: | 27.75% |
| Primary researcher: | Francoise Gondois-Rey | PI/manager: | Francoise Gondois-Rey | Uploaded by: | Francoise Gondois-Rey |
| Experiment dates: | 2011-05-05 - | Dataset uploaded: | Sep 2014 | Last updated: | Sep 2014 |
| Keywords: | [multi-parametric flow cytometry] [automated analysis] [population annotation with heatmaps] | Manuscripts: | |||
| Organizations: |
Centre de Recherche en Cancérologie de Marseille, Marseille, (France)
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| Purpose: | Exploration of innate immune cells populations in PBMC | ||||
| Conclusion: | 7 populations involved in innate immunity were identified : NKbright, NKdim, classical monocytes, CD16+ monocytes, CD16+DC, myeloid DC, plasmacytoid DC by manual analysis, among the 5 additionnal populations identified by computation of the data, 3 were identified as basophils, CD56-CD16+NK, activated monocytes, the 2 remaining were negative for all markers, or identified as debris | ||||
| Comments: | None | ||||
| Funding: | Research was funded by an Institut National du Cancer - Institut National de la Santé et de la Recherche Médicale grant to GB. Support for hardware was obtained from Fondation pour la Recherche Médicale and Aix-Marseille BQR Preciput grants to GB. PR was funded by an Aix-Marseille Univ. postdoctoral Fellowship. DO laboratory is supported by the Fondation pour la Recherche Médicale (Equipe FRM DEQ20140329534). DO is a scholar of the Institut Universitaire de France. | ||||
| Quality control: | Cytometer was standardized with CST beads. PMT were set above the lower limit using unstained and fully stained sample | ||||
