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Experiment Overview

Repository ID: FR-FCM-ZZGC Experiment name: SOPHE data MIFlowCyt score: 88.98%
Primary researcher: John Zaunders PI/manager: John Zaunders Uploaded by: John Zaunders
Experiment dates: 2014-01-19 - 2014-09-23 Dataset uploaded: Oct 2014 Last updated: Mar 2015
Keywords: [automated gating] [T cell] [lymphocyte subsets] Manuscripts: Cytalogo
Organizations: UNSW Australia, Kirby Institute, Kensington, NSW (Australia)
Purpose: Validate SOPHE clustering using beads and known populations compared with FlowJo manual gating results, up to 33 dimensions
Conclusion: Very close correlations at relatively low dimensions, with still good correlations at high dimensions, but becoming very difficult to directly compare results
Comments: None
Funding: NHMRC Australia
Quality control: Used defined mixtures of beads Compensation controls Replicate experiments were run
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