Experiment Overview
| Repository ID: | FR-FCM-ZZGM | Experiment name: | Standardization of microparticle counts | MIFlowCyt score: | 33.17% |
| Primary researcher: | Philippe Poncelet | PI/manager: | Philippe Poncelet | Uploaded by: | Philippe Poncelet |
| Experiment dates: | 2012-01-02 - 2013-02-26 | Dataset uploaded: | Oct 2014 | Last updated: | May 2015 |
| Keywords: | [microparticles] [Standardization] [FSC] [SSC] [Megamix] [inter platform reproducibility] | Manuscripts: |
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| Organizations: |
BioCytex, R&T, Marseille, France (France)
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| Purpose: | Develop a new methodology allowing reproducible counts of human platelet microparticles (from 0.3µm-eq. to 1µm-eq. in FSC or the corresponding levels in SSC) with different instruments that have different scatter capabilities (in terms of resolution of submicron beads and background rejection capacity). | ||||
| Conclusion: | Using 2 different standardization strategies, based on either SSC or FSC depending on the instrument most efficient size-related parameter, we obtained a good inter-platform equivalence of PMP counts. | ||||
| Comments: | see corresponding paper Poncelet et al submitted to Cytometry A in Nov 2014 | ||||
| Funding: | N.A. | ||||
| Quality control: | Megamix-Plus beads BioCytex, Marseille, F) to evaluate scatter resolution and define scatter settings and threshold. | ||||
