Experiment Overview
| Repository ID: | FR-FCM-ZZGN | Experiment name: | Liposome measurement | MIFlowCyt score: | 79.77% |
| Primary researcher: | John Nolan | PI/manager: | John Nolan | Uploaded by: | John Nolan |
| Experiment dates: | 2013-06-27 - 2014-11-03 | Dataset uploaded: | Nov 2014 | Last updated: | Oct 2015 |
| Keywords: | [liposomes] [vesicles] [di-8-ANEPPS] | Manuscripts: |
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| Organizations: |
Scintillon Institute, San Diego, CA (USA)
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| Purpose: | Measure the fluorescence intensity of di-8ANEPPS-stained liposomes of different sizes. | ||||
| Conclusion: | The fluorescence of di-8-ANEPPS-stained liposomes is proportional to liposome surface area. | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | Instrument performance was characterized using a combination of multi-intensity multifluorophore beads (Rainbow, Spherotech) and multi-intensity single fluorophore beads (Quantum FITC, Bangs) whose intensity had been calibrated in units of MESF. Linear regression of the bead MFI vs MESF was used to create a standard curve to assign MESF values to the multifluorophore beads measured under the same instrument conditions (laser power, filters, flow rates). The instrument performance was characterized in terms of Q (detection efficiency) and B (background), essentially as described by Hoffman(21). A spreadsheet model (27) was used to simulate expected intensity histograms from particles of defined intensity and in instrument with those Q and B values. The values Separation (28) and Separation Index (29) were calculated. | ||||
Experiment variables
| Sample Type | |
|---|---|
| · 200 nm pore | 20130627_14.fcs |
| · 100 nm pore | 20130627_16.fcs |
| · 80 nm pore | 20130627_18.fcs |
| · 50 nm pore | 20130627_20.fcs |
| · PBS only | 20130627_22.fcs |
