Experiment Overview
| Repository ID: | FR-FCM-ZZGR | Experiment name: | FRET efficiency standards | MIFlowCyt score: | 26.25% |
| Primary researcher: | Jakub Nedbal | PI/manager: | Jakub Nedbal | Uploaded by: | Jakub Nedbal |
| Experiment dates: | 2014-07-29 - 2014-07-29 | Dataset uploaded: | Nov 2014 | Last updated: | Nov 2014 |
| Keywords: | [FRET] [biosensor] [HEK293T] [fluorescence lifetime] [FRET standard] | Manuscripts: |
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| Organizations: |
King's College, London, (UK)
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| Purpose: | Ratiometric FRET analysis of FRET standards expressed in HEK293T cells to support experiments performed by fluorescence lifetime flow cytometry. | ||||
| Conclusion: | The analysis qualitatively confirmed the results obtained by fluorescence lifetime flow cytometry. | ||||
| Comments: | The up-to-date source code and filer are available on GitHub: https://github.com/jnedbal/ratiometric-fret and Matlab Central File Exchange https://www.mathworks.com/matlabcentral/fileexchange/48437-ratiometric-fret | ||||
| Funding: | The research was funded by grant C1519/A16463 to the Cancer research UK & EPSRC Cancer Imaging Centre at KCL & UCL, the Engineering and Physical Sciences Research Council (UK) for studentship for V.V. and a Dimbleby Cancer Care endowment fund to King’s College London. The authors acknowledge financial support from the Department of Health via the National Institute for Health Research (NIHR) comprehensive Biomedical Research Centre award to Guy's & St Thomas' NHS Foundation Trust in partnership with King's College London and King's College Hospital NHS Foundation Trust. | ||||
| Quality control: | None | ||||
