Experiment Overview
| Repository ID: | FR-FCM-ZZGX | Experiment name: | EVs in rat PRP | MIFlowCyt score: | 79.12% |
| Primary researcher: | John Nolan | PI/manager: | John Nolan | Uploaded by: | John Nolan |
| Experiment dates: | 2013-07-01 - 2014-05-01 | Dataset uploaded: | Nov 2014 | Last updated: | Oct 2015 |
| Keywords: | [Extracellular vesicles] [microvesicles] [micropartices] [rat] [platelets] [platelet-rich plasma] [PRP] | Manuscripts: |
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| Organizations: |
Scintillon Institute, San Diego, CA (USA)
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| Purpose: | Detect and phenotype extracellular vesicles in rat plasma. | ||||
| Conclusion: | High sensitivity flow cytometry using a fluoregenic membrane probe and fluorescent surface markers can be used to measure individual extracellular vesicles in plasma. | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | Instrument performance was characterized using a combination of multi-intensity multifluorophore beads (Rainbow, Spherotech) and multi-intensity single fluorophore beads (Quantum FITC, Bangs) whose intensity had been calibrated in units of MESF. Linear regression of the bead MFI vs MESF was used to create a standard curve to assign MESF values to the multifluorophore beads measured under the same instrument conditions (laser power, filters, flow rates). The instrument performance was characterized in terms of Q (detection efficiency) and B (background), essentially as described by Hoffman(21). A spreadsheet model (27) was used to simulate expected intensity histograms from particles of defined intensity and in instrument with those Q and B values. The values Separation (28) and Separation Index (29) were calculated. | ||||
Experiment variables
| Doses | |
|---|---|
| · 0 | 20140107_04.fcs · 20140107_06.fcs · 20140107_10.fcs · 20140107_26.fcs · 20140107_27.fcs |
| · 10 uM | 20140107_05.fcs · 20140107_08.fcs · 20140107_28.fcs |
