Experiment Overview
| Repository ID: | FR-FCM-ZZJ5 | Experiment name: | PSMS compensation T cell subpopulation panel | MIFlowCyt score: | 49.25% |
| Primary researcher: | Tiara Byrd | PI/manager: | Tiara Byrd | Uploaded by: | Tiara Byrd |
| Experiment dates: | 2013-03-07 - 2015-02-17 | Dataset uploaded: | Mar 2015 | Last updated: | Jul 2015 |
| Keywords: | [flow cytometry] [Immunophenotyping] [Beads] [Compensation] [Polystyrene Microspheres] [Polychromatic] [Leukocytes] | Manuscripts: |
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| Organizations: |
Baylor College of Medicine, Translational Biology and Molecular Medicine, Houston, (Texas)
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| Purpose: | To assess the use of polystyrene microspheres compared to the use of cell sample for single color based compensation for analysis of primary leukocytes using polychromatic flow cytometry | ||||
| Conclusion: | Polystyrene microspheres display similar autofluorescence to cells (negative peak). PSMS have a more even distribution of negative and positive events as well as discrete popualtions to use in compensation. PSMS based compensation did not yield any statistically significant differences in the data of three donors stained with our ten color T cell panel. | ||||
| Comments: | None | ||||
| Funding: | Research was supported by the Alliance for Cancer Gene Therapy (ACGT, Inc), Alex’s Lemonade Stand Pediatric Cancer Foundation (ALSF) and Stand Up To Cancer ¬ St. Baldrick¹s Pediatric Dream Team Translational Research Grant (SU2C-AACR-DT1113). Stand Up To Cancer is a program of the Entertainment Industry Foundation administered by the American Association for Cancer Research. This research is also supported in part by Award Numbers T32GM088129 and 5T32HL092332 from the National Institute Of General Medical Sciences and National Heart, Lung and Blood Institute, respectively. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institute Of General Medical Sciences, National Heart, Lung and Blood Institute or the National Institutes of Health. | ||||
| Quality control: | Polystyrene Microspheres Enable 10-color Compensation for Immunophenotyping of Primary Leukocytes in a Clinical Study | Here PSMS and PBMC were ran to set up compensation matrices to be used for analysis of three donors | |||
