Experiment Overview
| Repository ID: | FR-FCM-ZZJB | Experiment name: | Quantitation of Cell-Cell Fusion Using the Multicolor FCM Assay I | MIFlowCyt score: | 91.48% |
| Primary researcher: | Valentina Salzman | PI/manager: | Pablo Aguilar | Uploaded by: | Valentina Salzman |
| Experiment dates: | 2014-07-15 - 2014-07-15 | Dataset uploaded: | Mar 2015 | Last updated: | May 2015 |
| Keywords: | [fertilization] [mating] [bi-molecular complementation] [Saccharomyces cerevisiae] | Manuscripts: |
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| Organizations: |
Institut Pasteur de Montevideo, Montevideo, (Uruguay)
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| Purpose: | The purpose of the experiment is to quantify cell-cell fusion by flow cytometric analysis of a wt X wt and a prm1∆ X prm1∆ yeast mating. MATa and MATalpha strains are distinguished by staining each strain with ConcanavalinA- Alexa Fluor 647 (ConA-647) and with ConcanavalinA- Tetramethylrhodamine (ConA-Tet), respectively. Mating pairs are revealed as two-colored entities. Cytoplasmic mixing is measured with a GFP bi-molecular fluorescence complementation assay. Cell fusion efficiency is calculated as the percentage of fused mating pairs over the total number of pairs. | ||||
| Conclusion: | Cell fusion efficiencies of a wt X wt mating and a prm1∆ X prm1∆ mating were quantified. | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | Each mating sample was analyzed by triplicate, unstained and compensation controls are included in the experiment. | ||||
Experiment variables
| Sample Type | |
|---|---|
| · wt control samples | 1_15_Jul_14.fcs · 2_15_Jul_14.fcs |
| · prm1 control samples | 3_15_Jul_14.fcs · 4_15_Jul_14.fcs · 7_15_Jul_14.fcs · 9_15_Jul_14.fcs |
| · wt X wt mating samples | 11V_15_Jul_14.fcs · 12_15_Jul_14.fcs · 13_15_Jul_14.fcs |
| · prm1 X prm1 mating samples | 14_15_Jul_14.fcs · 15_15_Jul_14.fcs · 16_15_Jul_14.fcs |
