Experiment Overview
| Repository ID: | FR-FCM-ZZJH | Experiment name: | Quantitation of Cell-Cell Fusion Using the Multicolor FCM Assay III | MIFlowCyt score: | 90.00% |
| Primary researcher: | Valentina Salzman | PI/manager: | Pablo Aguilar | Uploaded by: | Valentina Salzman |
| Experiment dates: | 2014-05-27 - 2014-05-27 | Dataset uploaded: | Mar 2015 | Last updated: | May 2015 |
| Keywords: | [fertilization] [mating] [bi-molecular complementation] [Saccharomyces cerevisiae] | Manuscripts: |
|
||
| Organizations: |
Institut Pasteur de Montevideo, Montevideo, (Uruguay)
|
||||
| Purpose: | The purpose of the experiment is to quantify cell-cell fusion by flow cytometric analysis of a wt X wt and a prm1∆ X prm1∆ yeast mating. MATa and MATalpha strains are distinguished by staining each strain with ConcanavalinA- Alexa Fluor 647 (ConA-647) and with ConcanavalinA- Tetramethylrhodamine (ConA-Tet), respectively. Mating pairs are revealed as two-colored entities. Cytoplasmic mixing is measured with a GFP bi-molecular fluorescence complementation assay. Cell fusion efficiency is calculated as the percentage of fused mating pairs over the total number of pairs. | ||||
| Conclusion: | Cell fusion efficiencies of a wt X wt mating and a prm1∆ X prm1∆ mating were quantified. | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | Each mating sample was analyzed by triplicate, unstained and compensation controls are included in the experiment. | ||||
Experiment variables
| Sample Type | |
|---|---|
| · wt control samples | 1027_May_14.fcs · 127_May_14.fcs · 227_May_14.fcs · 627_May_14.fcs · 727_May_14.fcs |
| · prm1 control samples | 1127_May_14.fcs · 327_May_14.fcs · 427_May_14.fcs · 827_May_14.fcs · 927_May_14.fcs |
| · wt X wt mating samples | 1227_May_14.fcs · 13 no Ca27_May_14.fcs · 14 no Ca27_May_14.fcs |
| · prm1 X prm1 mating samples | 15 no Ca27_May_14.fcs · 16 no Ca27_May_14.fcs · 17 no Ca27_May_14.fcs |
