Experiment Overview
| Repository ID: | FR-FCM-ZZJM | Experiment name: | Quantitation of Cell-Cell Fusion of wt Sonicated Samples Using the Multicolor FCM Assay I | MIFlowCyt score: | 91.75% |
| Primary researcher: | Valentina Salzman | PI/manager: | Pablo Aguilar | Uploaded by: | Valentina Salzman |
| Experiment dates: | 2014-01-30 - 2014-01-30 | Dataset uploaded: | Mar 2015 | Last updated: | May 2015 |
| Keywords: | [fertilization] [mating] [bi-molecular complementation] [Saccharomyces cerevisiae] | Manuscripts: |
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| Organizations: |
Institut Pasteur de Montevideo, Montevideo, (Uruguay)
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| Purpose: | The purpose of the experiment is to quantify cell-cell fusion by flow cytometric analysis of a wt X wt. MATa and MATalpha strains are distinguished by staining each strain with ConcanavalinA- Alexa Fluor 647 (ConA-647) and with ConcanavalinA- Tetramethylrhodamine (ConA-Tet), respectively. Mating pairs are revealed as two-colored entities. Cytoplasmic mixing is measured with a GFP bi-molecular fluorescence complementation assay. Cell fusion efficiency is calculated as the percentage of fused mating pairs over the total number of pairs. Samples are sonicated before flow cytometry analysis. Results are compared with results obtained with non sonicated samples. | ||||
| Conclusion: | Cell fusion efficiencies of sonicated and non sonicated wt X wt matings were similar. | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | Each mating sample was analyzed by triplicate, sonicated unstained and compensation controls are included in the experiment. | ||||
Experiment variables
| Sample Type | |
|---|---|
| · wt control samples | S 1 150m DPBS _30_Jan_14.fcs · S 2 150m DPBS _30_Jan_14.fcs · S 3 150m DPBS _30_Jan_14.fcs · S 4 150m DPBS _30_Jan_14.fcs · S 5 150m DPBS _30_Jan_14.fcs |
| · wt X wt mating samples | S 6 150m DPBS _30_Jan_14.fcs · S 7 150m DPBS _30_Jan_14.fcs · S 8 150m DPBS _30_Jan_14.fcs |
