Experiment Overview
| Repository ID: | FR-FCM-ZZJP | Experiment name: | Quantitation of Cell-Cell Fusion of wt Non Sonicated Samples Using the Multicolor FCM Assay I | MIFlowCyt score: | 91.75% |
| Primary researcher: | Valentina Salzman | PI/manager: | Pablo Aguilar | Uploaded by: | Valentina Salzman |
| Experiment dates: | 2014-01-30 - 2014-01-30 | Dataset uploaded: | Mar 2015 | Last updated: | May 2015 |
| Keywords: | [fertilization] [mating] [bi-molecular complementation] [Saccharomyces cerevisiae] | Manuscripts: |
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| Organizations: |
Institut Pasteur de Montevideo, Montevideo, (Uruguay)
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| Purpose: | The purpose of the experiment is to quantify cell-cell fusion by flow cytometric analysis of a wt X wt. MATa and MATalpha strains are distinguished by staining each strain with ConcanavalinA- Alexa Fluor 647 (ConA-647) and with ConcanavalinA- Tetramethylrhodamine (ConA-Tet), respectively. Mating pairs are revealed as two-colored entities. Cytoplasmic mixing is measured with a GFP bi-molecular fluorescence complementation assay. Cell fusion efficiency is calculated as the percentage of fused mating pairs over the total number of pairs. | ||||
| Conclusion: | Cell fusion efficiency of a wt X wt non sonicated mating was similar to sonicated samples of experiment titled Quantitation of Cell-Cell Fusion of wt Sonicated Samples Using the Multicolor FCM Assay I. | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | Unstained and compensation controls were included in the experiment, matings were analyzed by triplicate. | ||||
Experiment variables
| Sample Type | |
|---|---|
| · wt control samples | 1 150m_30_Jan_14.fcs · 2 150m_30_Jan_14.fcs · 3 150m_30_Jan_14.fcs · 4 150m_30_Jan_14.fcs · 5 150m_30_Jan_14.fcs |
| · wt X wt mating samples | 6 150m_30_Jan_14.fcs · 7 150m_30_Jan_14.fcs · 8 150m_30_Jan_14.fcs |
