Experiment Overview
| Repository ID: | FR-FCM-ZZJT | Experiment name: | Maintenance ConA-fluorophore Label Under Standard Mating Conditions | MIFlowCyt score: | 87.75% |
| Primary researcher: | Valentina Salzman | PI/manager: | Pablo Aguilar | Uploaded by: | Valentina Salzman |
| Experiment dates: | 2013-12-13 - 2013-12-13 | Dataset uploaded: | Mar 2015 | Last updated: | May 2015 |
| Keywords: | [fertilization] [mating] [bi-molecular complementation] [Saccharomyces cerevisiae] | Manuscripts: |
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| Organizations: |
Institut Pasteur de Montevideo, Montevideo, (Uruguay)
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| Purpose: | The purpose of the experiment is to test if the ConA-fluorophore label is retained by the cells' surface under the standard mating conditions. Haploid-single-stained samples are obtained using optimized ConA-fluorophore conjugates concentrations, vacuum-filtered and incubated for 0.5, or 2.5 h in SD agar plates at 30ºC. | ||||
| Conclusion: | By comparing the median ConA-Tet fluorescence intensity of the ConA-Tet positive subpopulations we determined that the older cell wall components (that is, mother cells' walls) retained more than 65 % of the initial fluorescent label after the mating incubation period. | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | Unstained controls obtained at the different time points were included | ||||
Experiment variables
| Sample Type | |
|---|---|
| · Unstained | 1P30min_13_Dec_13.fcs · 1p2.fcs |
| · Stained | 3P30min_13_Dec_13.fcs · 3p2.fcs |
