Experiment Overview
| Repository ID: | FR-FCM-ZZKJ | Experiment name: | ROS in mycobacteria | MIFlowCyt score: | 51.50% |
| Primary researcher: | Megan McBee | PI/manager: | Megan McBee | Uploaded by: | Megan McBee |
| Experiment dates: | 2013-07-03 - 2013-08-18 | Dataset uploaded: | Jun 2015 | Last updated: | Jul 2024 |
| Keywords: | [Bacteria] [Mycobacteria] [reactive oxygen species] [starvation] | Manuscripts: | |||
| Organizations: |
Singapore-MIT Alliance for Research and Technology, Infectious Disease, Singapore, (Singapore)
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| Purpose: | Detection and evaluation of intracellular ROS generation in log growing and starved mycobacteria treated with antibiotics or hydrogen peroxide. | ||||
| Conclusion: | Some antibiotic stress leads to ROS generation in mycobacteria, as does starvation stress. However, hydrogen peroxide stress does not generate intracellular ROS in mycobacteria. | ||||
| Comments: | None | ||||
| Funding: | National Research Foundation, Singapore | ||||
| Quality control: | Single color stains were used for compensation each day experiments were conducted. Treatment controls were also used to set background fluorescence gates. | ||||
