Experiment Overview
| Repository ID: | FR-FCM-ZZKZ | Experiment name: | Diggins, Ferrell, and Irish - Methods for discovery and characterization of cell subsets in high dimensional mass cytometry data | MIFlowCyt score: | 34.08% |
| Primary researcher: | Kirsten Diggins | PI/manager: | Jonathan Irish | Uploaded by: | Kirsten Diggins |
| Experiment dates: | 2015-04-16 - 2015-04-16 | Dataset uploaded: | Apr 2015 | Last updated: | Jun 2015 |
| Keywords: | [mass cytometry] [machine learning] [computational biology] [viSNE] [SPADE] [heatmaps] | Manuscripts: | [25979346] [PMC4468028] | ||
| Organizations: |
Vanderbilt University, Cancer Biology, Nashville, TN (USA)
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| Purpose: | This experiment provides FCS data files, tables of statistics from SPADE, an R script, and a detailed protocol to facilitate data re-analysis and the use of the workflow found in Diggins et al. | ||||
| Conclusion: | This method combines multiple tools for high dimensional data analysis to allow for the semi-supervised discovery and characterization of cells identified by mass cytometry analysis. | ||||
| Comments: | No comments | ||||
| Funding: | P30 CA068485 - Cancer Center Support Grant K12 CA090625 - Vanderbilt Clinical Oncology Research Career Development Award R00 CA143231 - Targeting the B Cell Receptor Signaling Network in Lymphoma R25 CA136440 - Training in Cellular and Molecular Imaging of Cancer | ||||
| Quality control: | No quality control | ||||
Experiment variables
| Individuals | |
|---|---|
| · AML Patient 001 peripheral blood | AML_PB0.fcs |
| · AML Patient 001 peripheral blood viSNE gated blasts | AML_PB0_viSNEgate_Blasts.fcs |
| · AML Patient 001 peripheral blood viSNE gated non-Blasts | AML__PB0_viSNEgate_Nonblast.fcs |
| · Concatenated AML blood and normal marrow after viSNE gating | AML_normal_viSNEgates_concat.fcs |
| · Normal bone marrow | normal_marrow.fcs |
| · Normal bone marrow viSNE gated non-Blasts | normal_marrow_viSNEgate_Nonblast.fcs |
