Experiment Overview
| Repository ID: | FR-FCM-ZZL3 | Experiment name: | Kinin-B1 and B2 receptor in mouse embryonic stem cells | MIFlowCyt score: | 24.00% |
| Primary researcher: | Isis Nascimento | PI/manager: | Isis Nascimento | Uploaded by: | Isis Nascimento |
| Experiment dates: | 2012-09-01 - 2013-03-31 | Dataset uploaded: | Jul 2015 | Last updated: | Jul 2015 |
| Keywords: | [Embryonic stem cells] [Undifferentiated cell marker] [bradykinin] [neural differentiation] | Manuscripts: |
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| Organizations: | None | ||||
| Purpose: | The purpose of the experiment was to quantify the expression of neural differentiation markers during neural differentiation in dependence of kinin receptor activity | ||||
| Conclusion: | Analysis by flow cytometry showed that over 85% of the undifferentiated cells were positive for OCT4 expression, ensuring the embryonic character of the cell population. The expression of kinin receptors was detected in ES cells. Furthermore, it was observed involvement of B1 kinin receptors in the regulation of cell proliferation and neuronal maturation, while the B2 receptor activity is related with neural phenotype choice, promoting neurogenesis over gliogenesis | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | None | ||||
