Experiment Overview

Repository ID: FR-FCM-ZZLG Experiment name: Sort of C.suis-/dim/high cells MIFlowCyt score: 100.00%
Primary researcher: Tobias Kaeser PI/manager: Tobias Kaeser Uploaded by: Tobias Kaeser
Experiment dates: 2015-04-27 - 2015-04-29 Dataset uploaded: Jul 2015 Last updated: Jan 2016
Keywords: [FACS] [confocal microscopy] [Chlamydia suis] [Hep2] [cytospin] Manuscripts: Cytalogo
Organizations: University of Saskatchewan, VIDO-InterVac, Saskatoon, SK (Canada)
Purpose: Sort C.suis-neg/dim/high population to cytospin them and analyse the cells in confocal microscopy. This shall show in which cell population the C. suis inclusion-positive cells are located.
Conclusion: C.suis-inclusion-positive cells are only in the C.suis-high subpopulation.
Comments: None
Funding: SHRF establishment grant
Quality control: Setup using Spherotec QC beads.

Experiment variables

· Pre-sort (non-infected) Presort Csuis Negative.fcs
· Pre-sort (infected) Presort Csuis Positive.fcs
· Post-sort (C. suis-neg) C suis Neg resorted.fcs
· Post-sort (C. suis-dim) C suis low resorted.fcs
· Post-sort (C. suis-high) C suis high resorted.fcs

· 30hpi C suis Neg resorted.fcs · C suis high resorted.fcs · C suis low resorted.fcs · Presort Csuis Negative.fcs · Presort Csuis Positive.fcs

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