Experiment Overview
| Repository ID: | FR-FCM-ZZLG | Experiment name: | Sort of C.suis-/dim/high cells | MIFlowCyt score: | 100.00% |
| Primary researcher: | Tobias Kaeser | PI/manager: | Tobias Kaeser | Uploaded by: | Tobias Kaeser |
| Experiment dates: | 2015-04-27 - 2015-04-29 | Dataset uploaded: | Jul 2015 | Last updated: | Jan 2016 |
| Keywords: | [FACS] [confocal microscopy] [Chlamydia suis] [Hep2] [cytospin] | Manuscripts: |
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| Organizations: |
University of Saskatchewan, VIDO-InterVac, Saskatoon, SK (Canada)
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| Purpose: | Sort C.suis-neg/dim/high population to cytospin them and analyse the cells in confocal microscopy. This shall show in which cell population the C. suis inclusion-positive cells are located. | ||||
| Conclusion: | C.suis-inclusion-positive cells are only in the C.suis-high subpopulation. | ||||
| Comments: | None | ||||
| Funding: | SHRF establishment grant | ||||
| Quality control: | Setup using Spherotec QC beads. | ||||
Experiment variables
| Conditions | |
|---|---|
| · Pre-sort (non-infected) | Presort Csuis Negative.fcs |
| · Pre-sort (infected) | Presort Csuis Positive.fcs |
| · Post-sort (C. suis-neg) | C suis Neg resorted.fcs |
| · Post-sort (C. suis-dim) | C suis low resorted.fcs |
| · Post-sort (C. suis-high) | C suis high resorted.fcs |
| Timepoints | |
|---|---|
| · 30hpi | C suis Neg resorted.fcs · C suis high resorted.fcs · C suis low resorted.fcs · Presort Csuis Negative.fcs · Presort Csuis Positive.fcs |
