Experiment Overview
| Repository ID: | FR-FCM-ZZLK | Experiment name: | Heparin blocking to prevent non-specific CyTOF antibody binding by eosinophils | MIFlowCyt score: | 19.50% |
| Primary researcher: | Adeeb Rahman | PI/manager: | Adeeb Rahman | Uploaded by: | Adeeb Rahman |
| Experiment dates: | 2015-05-01 - | Dataset uploaded: | Jul 2015 | Last updated: | Jul 2015 |
| Keywords: | None | Manuscripts: | [27061608] |
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| Organizations: | None | ||||
| Purpose: | To demonstrate that human blood eosinophils non-specifically bind to CyTOF Maxpar antibodies following fixation and permeabilization of whole blood samples, and that this non-specific binding can be prevented by blocking with heparin. | ||||
| Conclusion: | None | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | None | ||||
Experiment variables
| Sample Type | |
|---|---|
| · Pre-fixation | 150501_Heptest_pre_1.fcs |
| · post-BD lyse/fix | 150501_Heptest_post_0_1.fcs · 150501_Heptest_post_100_1.fcs · 150501_Heptest_post_10_1.fcs · 150501_Heptest_post_333_1.fcs · 150501_Heptest_post_33_1.fcs · 150501_Heptest_post_3_1.fcs |
| · post-Triton X-100 | 150501_Heptest_post_TX100_0_1.fcs · 150501_Heptest_post_TX100_100_1.fcs · 150501_Heptest_post_TX100_10_1.fcs · 150501_Heptest_post_TX100_333_1.fcs · 150501_Heptest_post_TX100_33_1.fcs · 150501_Heptest_post_TX100_3_1.fcs |
| Doses | |
|---|---|
| · 0 | 150501_Heptest_post_0_1.fcs · 150501_Heptest_post_TX100_0_1.fcs · 150501_Heptest_pre_1.fcs |
| · 3 | 150501_Heptest_post_3_1.fcs · 150501_Heptest_post_TX100_3_1.fcs |
| · 10 | 150501_Heptest_post_10_1.fcs · 150501_Heptest_post_TX100_10_1.fcs |
| · 33 | 150501_Heptest_post_33_1.fcs · 150501_Heptest_post_TX100_33_1.fcs |
| · 100 | 150501_Heptest_post_100_1.fcs · 150501_Heptest_post_TX100_100_1.fcs |
| · 333 | 150501_Heptest_post_333_1.fcs · 150501_Heptest_post_TX100_333_1.fcs |
