Experiment Overview
| Repository ID: | FR-FCM-ZZM4 | Experiment name: | DNA ploidy analysis in B-ALL MRD using FxCycle violet | MIFlowCyt score: | 84.00% |
| Primary researcher: | prashant tembhare | PI/manager: | prashant tembhare | Uploaded by: | prashant tembhare |
| Experiment dates: | 2014-01-01 - 2014-12-31 | Dataset uploaded: | Aug 2015 | Last updated: | Dec 2015 |
| Keywords: | [Simultaneous six color surface staining & DNA ploidy ] [B-ALL] [MRD] | Manuscripts: |
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| Organizations: |
Tata Memorial Centre, Hematopathology Laboratory, Mumbai, MAHARASHTRA (India)
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| Purpose: | To demonstrate application of DNA ploidy analysis using Fxcycle violet (FCV) and simultaneous multicolor (4-7) surface immunophenotyping. | ||||
| Conclusion: | This method DNA ploidy analysis using FxCycle violet and simultaneous six color immunophenotyping can be used flow cytometric DNA ploidy determination in B-ALL MRD evaluation (up to 100 events). | ||||
| Comments: | This experiment proves that this method can be useful for DNA ploidy analysis for B-ALL MRD analysis. However, it is recommended acquire at least 200 events of tumor cells for good results for rare events of tumor. Although, CV of G1 peak might be slightly higher (between 4 to 6), it is acceptable as it is still falls within the 10% standard error and does not affect the results of the assay. Few unstained and apoptotic cells always present in the sample showing decreasing staining pattern of FxCycle. However, these does not interfere with analysis and interpretation once gated out. | ||||
| Funding: | Not disclosed | ||||
| Quality control: | DNA ploidy and simultaneous six color immunophenotyping using FxCycle-Violet. | Daily routine QC as per manufacturer's instructions and compensation (both generic and tube specific post-acquisition) was performed. Sample was processed within 12 hours of collection. Timing of fixation was strictly followed. | |||
Experiment variables
| Timepoints | |
|---|---|
| · Post induction (day 28) | B_ALL MRD_FCV DNA 1.LMD · Re_B_ALL MRD_FCV DNA 2.LMD |
| Sample Type | |
|---|---|
| · Bone Marrow Aspirate | B_ALL MRD_FCV DNA 1.LMD · Re_B_ALL MRD_FCV DNA 2.LMD |
