Experiment Overview
| Repository ID: | FR-FCM-ZZM5 | Experiment name: | Intra-assay variation experiment for DNA ploidy with FxCycle Violet and simulatneous surface immunophenotyping | MIFlowCyt score: | 84.00% |
| Primary researcher: | prashant tembhare | PI/manager: | prashant tembhare | Uploaded by: | prashant tembhare |
| Experiment dates: | 2014-01-01 - 2014-12-31 | Dataset uploaded: | Sep 2015 | Last updated: | Dec 2015 |
| Keywords: | [FxCycle Violet] [Simultaneous six color surface staining & DNA ploidy ] [Intra-assay variation] | Manuscripts: |
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| Organizations: |
Tata Memorial Centre, Hematopathology Laboratory, Mumbai, MAHARASHTRA (India)
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| Purpose: | To study the intra-assay variation in DNA ploidy analysis using FxCycle Violet and simultaneous multicolor immunophenotyping | ||||
| Conclusion: | The inta-assay variation of DNA ploidy analysis using FxCycle Violet and simultaneous multi-color immunophenotyping is almost negligible. | ||||
| Comments: | In this experiment, a peripheral blood sample is analyzed for intra-assay variation in DNA index of the cells studied using FxCycle Violet. The same sample is processed and acquired ten times and DNA index of CD19+ cells is calculated. | ||||
| Funding: | Not disclosed | ||||
| Quality control: | Sample is acquired on FACS Canto-II and daily QC was performed using CST beads. a generic compensation was applied during acquisition and tube specific compensation is done during analysis of data. approximately 10000-20000 events were acquired at low rate. Sample was processed within 12 hours of collection. Timing of fixation was strictly followed. | ||||
Experiment variables
| Sample Type | |
|---|---|
| · Peripheral blood | B_ALL_DNA IS_FX 01.LMD · B_ALL_DNA IS_FX 02.LMD · B_ALL_DNA IS_FX 03.LMD · B_ALL_DNA IS_FX 04.LMD · B_ALL_DNA IS_FX 05.LMD · B_ALL_DNA IS_FX 06.LMD · B_ALL_DNA IS_FX 07.LMD · B_ALL_DNA IS_FX 08.LMD · B_ALL_DNA IS_FX 09.LMD · B_ALL_DNA IS_FX 10.LMD |
| Timepoints | |
|---|---|
| · At diagnosis | B_ALL_DNA IS_FX 01.LMD · B_ALL_DNA IS_FX 02.LMD · B_ALL_DNA IS_FX 03.LMD · B_ALL_DNA IS_FX 04.LMD · B_ALL_DNA IS_FX 05.LMD · B_ALL_DNA IS_FX 06.LMD · B_ALL_DNA IS_FX 07.LMD · B_ALL_DNA IS_FX 08.LMD · B_ALL_DNA IS_FX 09.LMD · B_ALL_DNA IS_FX 10.LMD |
