Experiment Overview
| Repository ID: | FR-FCM-ZZNA | Experiment name: | Cooling water ecosystem (SCK-CEN) | MIFlowCyt score: | 76.00% |
| Primary researcher: | Ruben Props | PI/manager: | Ruben Props | Uploaded by: | Ruben Props |
| Experiment dates: | 2014-11-17 - 2015-03-10 | Dataset uploaded: | Nov 2015 | Last updated: | Nov 2015 |
| Keywords: | [microbial communities] [microbiome resource management] [microbiology] | Manuscripts: |
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| Organizations: | None | ||||
| Purpose: | High-frequency flow cytometry data was captured of a microbial ecosystem in order to track the community dynamics by a newly developed bioinformatic pipeline. The results were put in juxtaposition of 16S rRNA gene amplicon sequencing. | ||||
| Conclusion: | Alpha diversity metrics derived from flow cytometry and 16S rRNA gene amplicon sequencing provide identical interpretation of microbial community dynamics. | ||||
| Comments: | None | ||||
| Funding: | Funded by SCK-CEN. | ||||
| Quality control: | All analysis were conducted in technical replicates (n = 3). Machine performance was checked daily with fluorescent calibration beads. | ||||
