Experiment Overview
| Repository ID: | FR-FCM-ZZPA | Experiment name: | Exploiting single-cell quantitative data to map genetic variants having probabilistic effects | MIFlowCyt score: | 27.00% |
| Primary researcher: | Magali Richard | PI/manager: | Gael Yvert | Uploaded by: | Magali Richard |
| Experiment dates: | 2014-03-01 - 2015-05-31 | Dataset uploaded: | Feb 2016 | Last updated: | Feb 2016 |
| Keywords: | [Yeast] [galactose] [GAL] [dynamic gene expression] [GFP reporter] [DOT6 ] | Manuscripts: | [27479122] [PMC4968810] |
|
|
| Organizations: | None | ||||
| Purpose: | The purpose of the experiment is to quantify the dynamic of transcriptional response to galactose exposure by flow cytometric analysis of single yeast cells expressing a GFP reporter system. We aim i) to phenotype BY x RM segregants and to detect scPTLs for the cellular response to galactose and ii) to analyse cellular response to galactose in DOT6 allele replacement experiment. | ||||
| Conclusion: | i) The BY strain showed a gradual increase of expression through time that was relatively homogeneous among the cells, whereas the RM strain showed elevated cell-cell heterogeneity at intermediate activation time points We acquired the response of 61 meiotic segregants of the BY x RM cross. The procedure identified a locus on chromosome V that was highly significant at 30 minutes post induction. The locus was also significant at times 20 min and 40 min post induction. ii) Strains BY and BY-DOT6RM (isogenic to BY except for the DOT6 gene which was replaced by the RM allele) displayed very similar transcriptional responses at intermediate times of induction. | ||||
| Comments: | All the informations regarding samples are contained in the annos.csv file. Date corresponds to the date the experiment was performed. Strain corresponds to different yeast strains. Time corresponds to the time of galactose induction. Plate corresponds to the plate ID for a single experiment. Well corresponds to the well ID. Flow cytometry analysis was performed i) from March to April 2014 to phenotype BY x RM segregants and to detect scPTLs for the cellular response to galactose - experiment 1 : 20140327, - experiment 2 : 20140331, - experiment 3 : 20140403, - experiment 4 : 20140417, And ii) in May 2015 to analyse cellular response to galactose in DOT6 allele replacement experiment. | ||||
| Funding: | Not disclosed | ||||
| Quality control: | i) Controls GY221 and GY225 were included in each experiment ii) Controls GY1566 and GY1567 were included in each experiment | ||||
