Experiment Overview
| Repository ID: | FR-FCM-ZZQ7 | Experiment name: | WGA488 HEK293 staining | MIFlowCyt score: | 92.42% |
| Primary researcher: | Alan Stern | PI/manager: | Alan Stern | Uploaded by: | Alan Stern |
| Experiment dates: | 2015-07-30 - 2015-07-30 | Dataset uploaded: | Mar 2016 | Last updated: | Oct 2016 |
| Keywords: | [HEK293 cells] [WGA] [wheat germ agglutinin ] | Manuscripts: | [27768827] |
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| Organizations: |
Icahn School of Medicine at Mount Sinai , New York, NY (United States of America)
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| Purpose: | The purpose of this experiment is to determine if alexa fluor 488 conjugated wheat germ agglutinin (WGA) staining intensity is a suitable metric of cell size. Here we stain HEK293 cells with two different concentrations of alexa fluor 488 conjugated WGA. | ||||
| Conclusion: | For HEK293 cells, we have determined that there is a statistically significant correlation between FSC and WGA intensities, whereas there was no correlation in unstained cells. | ||||
| Comments: | Optimal WGA staining concentrations need to be determined for different cell lines. Variations in staining can also be due to differences in cell membrane components that bind WGA. | ||||
| Funding: | Funding was provided by the Icahn School of Medicine at Mount Sinai and the NIH Grants P50GM071558 (Systems Biology Center New York), R21CA196418, R01GM104184, U54HG008098 (LINCS Center), and U24AI118644 and T32 GM062754. | ||||
| Quality control: | Unstained HEK293 cells were used as the staining control population | ||||
Experiment variables
| Conditions | |
|---|---|
| · Mock | Specimen_001_mh1_007.fcs |
| · 10ug/mL WGA | Specimen_001_10ug h1_011.fcs · Specimen_001_10ug h2_012.fcs |
| · 1ug/mL WGA | Specimen_001_1ug h1_009.fcs · Specimen_001_1ug h2_010.fcs |
