Experiment Overview
| Repository ID: | FR-FCM-ZZR6 | Experiment name: | FRET automatic program | MIFlowCyt score: | 51.50% |
| Primary researcher: | Kerstin von Kolontaj | PI/manager: | Kerstin von Kolontaj | Uploaded by: | Kerstin von Kolontaj |
| Experiment dates: | 2014-07-31 - 2015-07-22 | Dataset uploaded: | May 2016 | Last updated: | Sep 2016 |
| Keywords: | [T cells] [FRET] [T cell activation] | Manuscripts: |
|
||
| Organizations: |
Miltenyi Biotec, R&D Engineering, Bergisch Gladbach, (Germany)
|
||||
| Purpose: | Development of an automated FRET-based system for the detection of molecular associations in cells on the MACSQuant flow cytometer | ||||
| Conclusion: | T cell activation for PBMC from of healthy blood donors could be measured via CD3 homoclustering and CD3-CD4 clustering using the automated FRET assay. | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | Routine calibration using MACSQuant calibration beads (Miltenyi Biotec) | ||||
