Experiment Overview
| Repository ID: | FR-FCM-ZZRJ | Experiment name: | Dilution series 40nm SA-AgNP on CD8-bio | MIFlowCyt score: | 77.25% |
| Primary researcher: | Axel Schulz | PI/manager: | Axel Schulz | Uploaded by: | Axel Schulz |
| Experiment dates: | 2016-03-01 - 2016-03-02 | Dataset uploaded: | May 2016 | Last updated: | Mar 2019 |
| Keywords: | [nanoparticle] [Titration] [mass cytometry] | Manuscripts: | [27351740] | ||
| Organizations: |
German Rheumatism Research Center Berlin, a Leibniz-Institute (DRFZ), Immunemonitoring, Berlin, (Germany)
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| Purpose: | Determine optimal concentration for usage of 40 nm SA-AgNP in mass cytometry Done on PBMC with CD8-bio as primary antibody Repetition of an older experiment for publication | ||||
| Conclusion: | Best signal with conc 1:25, but problems in all other channels (incomplete ionization, due to high metal content? (1:25 - 1:100). Best signal without this issue: 1:200. No sufficient resolution at titers above 1:400 | ||||
| Comments: | see Fig 1 D-E | ||||
| Funding: | Not disclosed | ||||
| Quality control: | Comparison to direct detection by CD8-168Er (serves also as no primary ab control) | ||||
