Experiment Overview
| Repository ID: | FR-FCM-ZZRL | Experiment name: | Dilution series 10nm SA-AgNP on CD8-bio | MIFlowCyt score: | 77.33% |
| Primary researcher: | Axel Schulz | PI/manager: | Axel Schulz | Uploaded by: | Axel Schulz |
| Experiment dates: | 2016-01-26 - 2016-01-27 | Dataset uploaded: | May 2016 | Last updated: | Mar 2019 |
| Keywords: | [nanoparticle] [Titration] [mass cytometry] | Manuscripts: | [27351740] | ||
| Organizations: |
German Rheumatism Research Center Berlin, a Leibniz-Institute (DRFZ), Immunemonitoring, Berlin, (Germany)
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| Purpose: | Determine optimal concentration for usage of 10 nm SA-AgNP in mass cytometry Done on PBMC with CD8-bio as primary antibody. Samples acquired as a CD45-barcoded convolute. | ||||
| Conclusion: | Best separation at dilution 1:25 | ||||
| Comments: | Samples with 40 nm SA-AgNP omitted from analysis because of problems in deconvolution due to low CD45 signal caused by high AgNP content (Problems in ionization of these cells) - for 40 nm AgNP --> repetition experiment (see FR-FCM-ZZRJ) | ||||
| Funding: | Not disclosed | ||||
| Quality control: | Comparison to direct detection by CD8-168Er (serves also as no primary ab control) | ||||
