Experiment Overview
| Repository ID: | FR-FCM-ZZRS | Experiment name: | Phenotyping of CD1c+ isolated PBMCs | MIFlowCyt score: | 67.75% |
| Primary researcher: | Lisa Richter | PI/manager: | Lisa Richter | Uploaded by: | Lisa Richter |
| Experiment dates: | 2014-08-05 - 2014-08-05 | Dataset uploaded: | Jun 2016 | Last updated: | Jun 2016 |
| Keywords: | [monocyte] [myeloid dendritic cells] [CD1c] [dendritic cells] | Manuscripts: | |||
| Organizations: |
Oslo University Hospital, Department of Pathology, Oslo, Norwegen (Norge)
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| Purpose: | To phenotype cells that are isolated when applying the "CD1c+ DC isolation kit" to PBMCs. We used CD45, HLA-DR, CD11c, CD14, SIRPa and CD1c | ||||
| Conclusion: | The CD1c+ fraction contains CD11c+CD14-SIRPa+ DCs, CD14+SIRPa+ monocytes as well as a minor fraction of DR-CD11c- cells (presumably T cells according to our other experiments). As seen in earlier work, CD1a and CD103 are not expressed by blood MNPs. | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | Cytometer performance and tracking was performed the same day. Beads were used for compensation (OneComp eBeads, eBioscience) | ||||
Experiment variables
| Individuals | |
|---|---|
| · donor 1 | isolated_donor 1 negative fraction_009.fcs · isolated_donor 1 positive fraction_010.fcs · native_donor 1_001.fcs |
| · donor 2 | isolated_donor 2 negative fraction_012.fcs · isolated_donor 2 positive fraction_011.fcs · native_donor 2_002.fcs |
| Sample Type | |
|---|---|
| · native | native_donor 1_001.fcs · native_donor 2_002.fcs |
| · positive fraction | isolated_donor 1 positive fraction_010.fcs · isolated_donor 2 positive fraction_011.fcs |
| · negative fraction (depleted for CD19) | isolated_donor 1 negative fraction_009.fcs · isolated_donor 2 negative fraction_012.fcs |
| · cultured CD1c+ fraction | isolated_donor 1 RPMI_001.fcs · isolated_donor 2 RPMI_002.fcs |
| Timepoints | |
|---|---|
| · cultured 48h | isolated_donor 1 RPMI_001.fcs · isolated_donor 2 RPMI_002.fcs |
