Experiment Overview
| Repository ID: | FR-FCM-ZZRW | Experiment name: | SA-AgNP and CD294 | MIFlowCyt score: | 76.00% |
| Primary researcher: | Axel Schulz | PI/manager: | Axel Schulz | Uploaded by: | Axel Schulz |
| Experiment dates: | 2016-05-24 - 2016-05-25 | Dataset uploaded: | May 2016 | Last updated: | Mar 2019 |
| Keywords: | [nanoparticle] [mass cytometry] | Manuscripts: | [27351740] | ||
| Organizations: |
German Rheumatism Research Center Berlin, a Leibniz-Institute (DRFZ), Immunemonitoring, Berlin, (Germany)
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| Purpose: | Detection of CD294 on CD4+ T cells, basophils, eosinophils by indirect staining with 40 nm SA-AgNP and mass cytometry in lysed WB | ||||
| Conclusion: | Detection successful and specific with CD294-bio + 40 nm SA-AgNP. Control without CD294-bio showed very low background. Control with CD294-163Dy achieved similar population frequencies as detection by SA-AgNP | ||||
| Comments: | See Fig S4A | ||||
| Funding: | Not disclosed | ||||
| Quality control: | -no primary antibody control (=no CD294-bio) -detection by CD294-163Dy (both controls are done in one sample) | ||||
