Experiment Overview
| Repository ID: | FR-FCM-ZZRX | Experiment name: | SA-AgNP and CD25 | MIFlowCyt score: | 77.50% |
| Primary researcher: | Axel Schulz | PI/manager: | Axel Schulz | Uploaded by: | Axel Schulz |
| Experiment dates: | 2015-12-07 - 2016-02-09 | Dataset uploaded: | May 2016 | Last updated: | Mar 2019 |
| Keywords: | [nanoparticle] [Treg] [CD25] [mass cytometry] | Manuscripts: | [27351740] | ||
| Organizations: |
German Rheumatism Research Center Berlin, a Leibniz-Institute (DRFZ), Immunemonitoring, Berlin, (Germany)
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| Purpose: | Evaluate and compare indirect detection of CD25 on T cells by SA-AgNP in mass cytometry | ||||
| Conclusion: | The titration revealed an optimal concentration of 40 nm SA-AgNP for detection of CD25-bio of 1:25. Signal intensities achieved by 40 nm SA-AgNP on CD25-bio were higher than direct detection by CD25-149Sm or indirect detection by anti-bio-165Ho. This allowed not only a distinct and specific discrimination of CD25hiCD127− regulatory T cells (Treg), but also improved the separation of CD25loCD127+ cells from the CD25–CD127lo population within CD4+ T cells and enabled the discrimination of CD25-expressing B cells | ||||
| Comments: | see Fig. 3; Fig. S5 | ||||
| Funding: | Not disclosed | ||||
| Quality control: | no primary antibody controls comparison to direct detection (CD25-149Sm) or indirect detection (anti-bio-165Ho) | ||||
