Experiment Overview
| Repository ID: | FR-FCM-ZZSQ | Experiment name: | In depth comparative phenotyping of blood innate myeloid leukocytes from healthy humans and macaques using mass cytometry_Human-dataset | MIFlowCyt score: | 27.00% |
| Primary researcher: | Jamila Younes | PI/manager: | Jamila Younes | Uploaded by: | Jamila Younes |
| Experiment dates: | 2016-07-11 - | Dataset uploaded: | Jul 2016 | Last updated: | Apr 2017 |
| Keywords: | [whole blood] [human] [mass cytometry] [CyTOF] [Leukocytes] [innate myeloid immunity] [macaque] | Manuscripts: | [28444973] |
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| Organizations: |
CEA, Division of Immuno-Virology, DSV/iMETI, Fontenay-aux-Roses, (France)
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| Purpose: | To take advantage of the multiparameter capacities of mass cytometry to simultaneously analyze innate myeloid leukocytes in both humans and macaques with the aim of obtaining a global vision of the different cellular populations and setting the ground for future translational studies across human and NHP models in healthy and pathological situations | ||||
| Conclusion: | Correlation analysis designed to compare the phenotypes of human and macaque cell nodes and based on 16 markers, including 15 shared clustering markers and CD19 for humans or CD20 for macaques, revealed similarities and differences between staining patterns. Whether distinct staining profiles revealed lack of sufficient cross-reactivity of antibodies or are biological meaningful differences between primate species remains to be clarified. Our study certainly contributes to better assess the advantages and limits of the use of non-human primate in preclinical research. | ||||
| Comments: | None | ||||
| Funding: | Innovative Medicines Initiative: grant agreement n° 115308 BIOVACSAFE Agence Nationale de la Recherche: grant number: ANR-10-EQPX-02-01 Agence Nationale de la Recherche: grant number: ANR-11-INBS-000 Agence Nationale de la Recherche: grant number: ANR-10-LABX-77 | ||||
| Quality control: | None | ||||
