Experiment Overview
| Repository ID: | FR-FCM-ZZT6 | Experiment name: | 2016_Koff | MIFlowCyt score: | 30.50% |
| Primary researcher: | Magdalena Nauerth | PI/manager: | Dirk Busch | Uploaded by: | Matthias Schiemann |
| Experiment dates: | 2015-10-01 - 2015-12-31 | Dataset uploaded: | Jul 2016 | Last updated: | Aug 2016 |
| Keywords: | None | Manuscripts: |
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| Organizations: | None | ||||
| Purpose: | We recently developed a technology that allows to precisely determine the T cell receptor (TCR) ? ligand binding strength by reversibel MHC multimer staining and subsequent measurement of TCR-ligand dissociation rates via real time fluorescence microscopy. Unfortunately, this technology is very laborious and difficult to transfer to other laboratories. Therefore, we tested whether the basic principle of Koff rate measurements could be transferred to a flow cytometry-based acquisition platform. | ||||
| Conclusion: | None | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | None | ||||
