Experiment Overview
| Repository ID: | FR-FCM-ZZTJ | Experiment name: | Optimization of mass cytometry sample cryopreservation after staining | MIFlowCyt score: | 31.50% |
| Primary researcher: | Hermi Rizal Sumatoh | PI/manager: | Hermi Rizal Sumatoh | Uploaded by: | Hermi Rizal Sumatoh |
| Experiment dates: | 2016-02-01 - 2016-03-03 | Dataset uploaded: | Aug 2016 | Last updated: | Nov 2016 |
| Keywords: | [mass cytometry] [freezing] [cellular surface markers] [intracellular staining] | Manuscripts: | [27798817] |
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| Organizations: |
Agency for Science, Technology and Research, Singapore Immunology Network, Singapore, (Singapore)
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| Purpose: | This report describes a strategy to cryopreserve stained cells in hope of alleviating numerous technical limitations with current sample-handling approaches. Here we evaluated two protocols of freezing at -80oC in 10% DMSO in FBS and 100% methanol against standard refrigeration at 4oC in staining buffer. | ||||
| Conclusion: | We conclude that freezing already stained samples suspended in 10% DMSO in FBS is practical and efficient way to preserve samples when needed. | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | EQ Four Element Calibration Beads (Fluidigm Corporation, South San Francisco, CA) were added at a concentration of 1% prior to acquisition. | ||||
