Experiment Overview
| Repository ID: | FR-FCM-ZZTP | Experiment name: | Validating compatibility of OsO4 staining with surface and intracellular staining by mass cytometry | MIFlowCyt score: | 60.62% |
| Primary researcher: | Adeeb Rahman | PI/manager: | Adeeb Rahman | Uploaded by: | Adeeb Rahman |
| Experiment dates: | 2016-09-09 - 2016-09-09 | Dataset uploaded: | Sep 2016 | Last updated: | Oct 2016 |
| Keywords: | [CyTOF] [cell size] [oso4] | Manuscripts: | [27768827] |
|
|
| Organizations: |
Icahn School of Medicine at Mt. Sinai, New York, NY (10029)
|
||||
| Purpose: | To test compatibility of OsO4 labeling in a mass cytometry experiment involving surface staining, methanol permeabilization and intracellular phospho-staining. | ||||
| Conclusion: | OsO4 labeling can be incorporated either before or after surface/intracellualar staining and does not appreciably affect expression of any of the markers tested. | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | For quality control, the acquisition event rate was maintained under 400 events/s, and the EQ beads were confirmed to have a median Eu151 intensity of over 1000 to ensure appropriate mass sensitivity. | ||||
