Experiment Overview
| Repository ID: | FR-FCM-ZZTY | Experiment name: | Standardization and Quality Control for High-Dimensional Mass Cytometry Studies of Human Samples | MIFlowCyt score: | 55.50% |
| Primary researcher: | Katja Kleinsteuber | PI/manager: | Katja Kleinsteuber | Uploaded by: | Katja Kleinsteuber |
| Experiment dates: | 2015-03-09 - 2016-03-18 | Dataset uploaded: | Jul 2016 | Last updated: | Jul 2016 |
| Keywords: | [HIV] [bronchoalveolar lavage] [CyTOF; mass cytometry; flow cytometry; standardization] [human immunology] | Manuscripts: |
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| Organizations: |
Ragon Institute of MGH, MIT and Harvard, Cambridge, MA (United States)
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| Purpose: | FCS files from three repeat-experiments to demonstrate mass cytometry standardization and quality control using a reference sample, as shown in the manuscript: "Standardization and Quality Control for High-Dimensional Mass Cytometry Studies of Human Samples" by Kleinsteuber et al (2016). | ||||
| Conclusion: | Spiking a consistent reference sample (in this case PBMCs from a healthy blood donor) allows quality control of experimental conditions and results in highly reproducible population frequencies through a robust gating strategy based on negative populations of the reference sample. | ||||
| Comments: | Tethered gating, as shown in Kleinsteuber et al (2016) has been applied using FlowJo. | ||||
| Funding: | U.S. National Institutes of Health (NIH) Scripps Center for HIV/AIDS Vaccine Immunology and Immunogen Discovery grant (CHAVI-ID and 1UM1-AI100663) to BDW; the Harvard University Center for AIDS Research (CFAR), an NIH-funded program (P30 AI060354) which is supported by NIH co-funding and participating institutes and centers, including the NIAID, NCI, NICHD, NHLBI, NIDA, NIMH, NIA, FIC, and OAR to BDW; by the U.S. National Institute of Allergy and Infectious Diseases (5U01HL121827-03) to BC, JLC, BDM and DK and by the European Research Council Marie Curie fellowship to KK (#628359). | ||||
| Quality control: | Reference sample spiked into each patient sample. | ||||
