Experiment Overview
| Repository ID: | FR-FCM-ZZUB | Experiment name: | Figure.3.PBMC.Donor2 | MIFlowCyt score: | 96.15% |
| Primary researcher: | John Nash | PI/manager: | John Nash | Uploaded by: | John Nash |
| Experiment dates: | 2015-02-13 - 2015-02-13 | Dataset uploaded: | Oct 2016 | Last updated: | Jan 2017 |
| Keywords: | [PBMC] [LPS] [TNF] [NFkB] | Manuscripts: | [28094900] |
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| Organizations: |
Stanford University, Stanford, CA (USA)
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| Purpose: | To interrogate the nuclear import of NFkB after TNFa and LPS treatment across different subsets within healthy human PBMCs. This is the second of two donors to be used for this purpose. | ||||
| Conclusion: | NFkB nuclear localization after TNFa and LPS treatment differed across cell subsets. | ||||
| Comments: | None | ||||
| Funding: | Cancer Biology Training Grant 5T32CA9302-35 | ||||
| Quality control: | There were three timepoints: 5, 15, and 30 minutes across two different NFkB-stimulatory ligands: TNFa and LPS. Each of these timepoints had an unstimulated control. All antibodies for this experiment were extensively titrated prior to use, along with SLA reagents. PBMCs were stimulated and processed fresh (as opposed to viably frozen). Negative controls included both unstimulated cells, and an SLA workup not using primary antibodies (no signal expected). The positive control was the interaction between double-strand DNA and histone H3 antibodies. | ||||
Experiment variables
| Conditions | |
|---|---|
| · Untreated | Specimen_001_A1_A01_001_Untreated.fcs · Specimen_001_A2_A02_002_Untreated.fcs · Specimen_001_A3_A03_003_Untreated.fcs · Specimen_001_B1_B01_006_Untreated.fcs · Specimen_001_B2_B02_007_Untreated.fcs · Specimen_001_B3_B03_008_Untreated.fcs · Specimen_001_C1_C01_011_Untreated.fcs · Specimen_001_C2_C02_012_Untreated.fcs · Specimen_001_C3_C03_013_Untreated.fcs · Specimen_001_D1_D01_016_Untreated.fcs · Specimen_001_D2_D02_017_Untreated.fcs · Specimen_001_D3_D03_018_Untreated.fcs · Specimen_001_E1_E01_021_Untreated.fcs · Specimen_001_E2_E02_022_Untreated.fcs · Specimen_001_E3_E03_023_Untreated.fcs · Specimen_001_F1_F01_026_Untreated.fcs · Specimen_001_F2_F02_027_Untreated.fcs · Specimen_001_F3_F03_028_Untreated.fcs |
| · TNFa | Specimen_001_A1_A01_001_TNFa.fcs · Specimen_001_A2_A02_002_TNFa.fcs · Specimen_001_A3_A03_003_TNFa.fcs · Specimen_001_B1_B01_006_TNFa.fcs · Specimen_001_B2_B02_007_TNFa.fcs · Specimen_001_B3_B03_008_TNFa.fcs · Specimen_001_C1_C01_011_TNFa.fcs · Specimen_001_C2_C02_012_TNFa.fcs · Specimen_001_C3_C03_013_TNFa.fcs · Specimen_001_D1_D01_016_TNFa.fcs · Specimen_001_D2_D02_017_TNFa.fcs · Specimen_001_D3_D03_018_TNFa.fcs · Specimen_001_E1_E01_021_TNFa.fcs · Specimen_001_E2_E02_022_TNFa.fcs · Specimen_001_E3_E03_023_TNFa.fcs · Specimen_001_F1_F01_026_TNFa.fcs · Specimen_001_F2_F02_027_TNFa.fcs · Specimen_001_F3_F03_028_TNFa.fcs |
| · LPS | Specimen_001_A1_A01_001_LPS.fcs · Specimen_001_A2_A02_002_LPS.fcs · Specimen_001_A3_A03_003_LPS.fcs · Specimen_001_B1_B01_006_LPS.fcs · Specimen_001_B2_B02_007_LPS.fcs · Specimen_001_B3_B03_008_LPS.fcs · Specimen_001_C1_C01_011_LPS.fcs · Specimen_001_C2_C02_012_LPS.fcs · Specimen_001_C3_C03_013_LPS.fcs · Specimen_001_D1_D01_016_LPS.fcs · Specimen_001_D2_D02_017_LPS.fcs · Specimen_001_D3_D03_018_LPS.fcs · Specimen_001_E1_E01_021_LPS.fcs · Specimen_001_E2_E02_022_LPS.fcs · Specimen_001_E3_E03_023_LPS.fcs · Specimen_001_F1_F01_026_LPS.fcs · Specimen_001_F2_F02_027_LPS.fcs · Specimen_001_F3_F03_028_LPS.fcs |
| Timepoints | |
|---|---|
| · 5min | Specimen_001_A1_A01_001_LPS.fcs · Specimen_001_A1_A01_001_TNFa.fcs · Specimen_001_A1_A01_001_Untreated.fcs · Specimen_001_B1_B01_006_LPS.fcs · Specimen_001_B1_B01_006_TNFa.fcs · Specimen_001_B1_B01_006_Untreated.fcs · Specimen_001_C1_C01_011_LPS.fcs · Specimen_001_C1_C01_011_TNFa.fcs · Specimen_001_C1_C01_011_Untreated.fcs · Specimen_001_D1_D01_016_LPS.fcs · Specimen_001_D1_D01_016_TNFa.fcs · Specimen_001_D1_D01_016_Untreated.fcs · Specimen_001_E1_E01_021_LPS.fcs · Specimen_001_E1_E01_021_TNFa.fcs · Specimen_001_E1_E01_021_Untreated.fcs · Specimen_001_F1_F01_026_LPS.fcs · Specimen_001_F1_F01_026_TNFa.fcs · Specimen_001_F1_F01_026_Untreated.fcs |
| · 15min | Specimen_001_A2_A02_002_LPS.fcs · Specimen_001_A2_A02_002_TNFa.fcs · Specimen_001_A2_A02_002_Untreated.fcs · Specimen_001_B2_B02_007_LPS.fcs · Specimen_001_B2_B02_007_TNFa.fcs · Specimen_001_B2_B02_007_Untreated.fcs · Specimen_001_C2_C02_012_LPS.fcs · Specimen_001_C2_C02_012_TNFa.fcs · Specimen_001_C2_C02_012_Untreated.fcs · Specimen_001_D2_D02_017_LPS.fcs · Specimen_001_D2_D02_017_TNFa.fcs · Specimen_001_D2_D02_017_Untreated.fcs · Specimen_001_E2_E02_022_LPS.fcs · Specimen_001_E2_E02_022_TNFa.fcs · Specimen_001_E2_E02_022_Untreated.fcs · Specimen_001_F2_F02_027_LPS.fcs · Specimen_001_F2_F02_027_TNFa.fcs · Specimen_001_F2_F02_027_Untreated.fcs |
| · 30min | Specimen_001_A3_A03_003_LPS.fcs · Specimen_001_A3_A03_003_TNFa.fcs · Specimen_001_A3_A03_003_Untreated.fcs · Specimen_001_B3_B03_008_LPS.fcs · Specimen_001_B3_B03_008_TNFa.fcs · Specimen_001_B3_B03_008_Untreated.fcs · Specimen_001_C3_C03_013_LPS.fcs · Specimen_001_C3_C03_013_TNFa.fcs · Specimen_001_C3_C03_013_Untreated.fcs · Specimen_001_D3_D03_018_LPS.fcs · Specimen_001_D3_D03_018_TNFa.fcs · Specimen_001_D3_D03_018_Untreated.fcs · Specimen_001_E3_E03_023_LPS.fcs · Specimen_001_E3_E03_023_TNFa.fcs · Specimen_001_E3_E03_023_Untreated.fcs · Specimen_001_F3_F03_028_LPS.fcs · Specimen_001_F3_F03_028_TNFa.fcs · Specimen_001_F3_F03_028_Untreated.fcs |
| Individuals | |
|---|---|
| · NPA | Specimen_001_A1_A01_001_LPS.fcs · Specimen_001_A1_A01_001_TNFa.fcs · Specimen_001_A1_A01_001_Untreated.fcs · Specimen_001_A2_A02_002_LPS.fcs · Specimen_001_A2_A02_002_TNFa.fcs · Specimen_001_A2_A02_002_Untreated.fcs · Specimen_001_A3_A03_003_LPS.fcs · Specimen_001_A3_A03_003_TNFa.fcs · Specimen_001_A3_A03_003_Untreated.fcs · Specimen_001_D1_D01_016_LPS.fcs · Specimen_001_D1_D01_016_TNFa.fcs · Specimen_001_D1_D01_016_Untreated.fcs · Specimen_001_D2_D02_017_LPS.fcs · Specimen_001_D2_D02_017_TNFa.fcs · Specimen_001_D2_D02_017_Untreated.fcs · Specimen_001_D3_D03_018_LPS.fcs · Specimen_001_D3_D03_018_TNFa.fcs · Specimen_001_D3_D03_018_Untreated.fcs |
| · dsDNA_H3 | Specimen_001_B1_B01_006_LPS.fcs · Specimen_001_B1_B01_006_TNFa.fcs · Specimen_001_B1_B01_006_Untreated.fcs · Specimen_001_B2_B02_007_LPS.fcs · Specimen_001_B2_B02_007_TNFa.fcs · Specimen_001_B2_B02_007_Untreated.fcs · Specimen_001_B3_B03_008_LPS.fcs · Specimen_001_B3_B03_008_TNFa.fcs · Specimen_001_B3_B03_008_Untreated.fcs · Specimen_001_E1_E01_021_LPS.fcs · Specimen_001_E1_E01_021_TNFa.fcs · Specimen_001_E1_E01_021_Untreated.fcs · Specimen_001_E2_E02_022_LPS.fcs · Specimen_001_E2_E02_022_TNFa.fcs · Specimen_001_E2_E02_022_Untreated.fcs · Specimen_001_E3_E03_023_LPS.fcs · Specimen_001_E3_E03_023_TNFa.fcs · Specimen_001_E3_E03_023_Untreated.fcs |
| · NFkB_dsDNA | Specimen_001_C1_C01_011_LPS.fcs · Specimen_001_C1_C01_011_TNFa.fcs · Specimen_001_C1_C01_011_Untreated.fcs · Specimen_001_C2_C02_012_LPS.fcs · Specimen_001_C2_C02_012_TNFa.fcs · Specimen_001_C2_C02_012_Untreated.fcs · Specimen_001_C3_C03_013_LPS.fcs · Specimen_001_C3_C03_013_TNFa.fcs · Specimen_001_C3_C03_013_Untreated.fcs · Specimen_001_F1_F01_026_LPS.fcs · Specimen_001_F1_F01_026_TNFa.fcs · Specimen_001_F1_F01_026_Untreated.fcs · Specimen_001_F2_F02_027_LPS.fcs · Specimen_001_F2_F02_027_TNFa.fcs · Specimen_001_F2_F02_027_Untreated.fcs · Specimen_001_F3_F03_028_LPS.fcs · Specimen_001_F3_F03_028_TNFa.fcs · Specimen_001_F3_F03_028_Untreated.fcs |
| Sample Type | |
|---|---|
| · Duplicate1 | Specimen_001_A1_A01_001_LPS.fcs · Specimen_001_A1_A01_001_TNFa.fcs · Specimen_001_A1_A01_001_Untreated.fcs · Specimen_001_A2_A02_002_LPS.fcs · Specimen_001_A2_A02_002_TNFa.fcs · Specimen_001_A2_A02_002_Untreated.fcs · Specimen_001_A3_A03_003_LPS.fcs · Specimen_001_A3_A03_003_TNFa.fcs · Specimen_001_A3_A03_003_Untreated.fcs · Specimen_001_B1_B01_006_LPS.fcs · Specimen_001_B1_B01_006_TNFa.fcs · Specimen_001_B1_B01_006_Untreated.fcs · Specimen_001_B2_B02_007_LPS.fcs · Specimen_001_B2_B02_007_TNFa.fcs · Specimen_001_B2_B02_007_Untreated.fcs · Specimen_001_B3_B03_008_LPS.fcs · Specimen_001_B3_B03_008_TNFa.fcs · Specimen_001_B3_B03_008_Untreated.fcs · Specimen_001_C1_C01_011_LPS.fcs · Specimen_001_C1_C01_011_TNFa.fcs · Specimen_001_C1_C01_011_Untreated.fcs · Specimen_001_C2_C02_012_LPS.fcs · Specimen_001_C2_C02_012_TNFa.fcs · Specimen_001_C2_C02_012_Untreated.fcs · Specimen_001_C3_C03_013_LPS.fcs · Specimen_001_C3_C03_013_TNFa.fcs · Specimen_001_C3_C03_013_Untreated.fcs |
| · Duplicate2 | Specimen_001_D1_D01_016_LPS.fcs · Specimen_001_D1_D01_016_TNFa.fcs · Specimen_001_D1_D01_016_Untreated.fcs · Specimen_001_D2_D02_017_LPS.fcs · Specimen_001_D2_D02_017_TNFa.fcs · Specimen_001_D2_D02_017_Untreated.fcs · Specimen_001_D3_D03_018_LPS.fcs · Specimen_001_D3_D03_018_TNFa.fcs · Specimen_001_D3_D03_018_Untreated.fcs · Specimen_001_E1_E01_021_LPS.fcs · Specimen_001_E1_E01_021_TNFa.fcs · Specimen_001_E1_E01_021_Untreated.fcs · Specimen_001_E2_E02_022_LPS.fcs · Specimen_001_E2_E02_022_TNFa.fcs · Specimen_001_E2_E02_022_Untreated.fcs · Specimen_001_E3_E03_023_LPS.fcs · Specimen_001_E3_E03_023_TNFa.fcs · Specimen_001_E3_E03_023_Untreated.fcs · Specimen_001_F1_F01_026_LPS.fcs · Specimen_001_F1_F01_026_TNFa.fcs · Specimen_001_F1_F01_026_Untreated.fcs · Specimen_001_F2_F02_027_LPS.fcs · Specimen_001_F2_F02_027_TNFa.fcs · Specimen_001_F2_F02_027_Untreated.fcs · Specimen_001_F3_F03_028_LPS.fcs · Specimen_001_F3_F03_028_TNFa.fcs · Specimen_001_F3_F03_028_Untreated.fcs |
