Experiment Overview
| Repository ID: | FR-FCM-ZZV7 | Experiment name: | Figure.S6.exp3 | MIFlowCyt score: | 95.75% |
| Primary researcher: | John Nash | PI/manager: | John Nash | Uploaded by: | John Nash |
| Experiment dates: | 2016-06-13 - 2016-06-13 | Dataset uploaded: | Oct 2016 | Last updated: | Jan 2017 |
| Keywords: | [DNA damage] [BRCA1] [Proximity Ligation Assay] [pH2AX] | Manuscripts: | [28009288] |
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| Organizations: |
Stanford University, Stanford, CA (USA)
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| Purpose: | To interrogate BRCA1:pH2AX interaction levels across the cell cycle in untreated cells and after ionizing radiation. | ||||
| Conclusion: | This interaction is significantly higher in S/G2 phase than G1. Ionizing radiation increases interaction primarily in G1. | ||||
| Comments: | None | ||||
| Funding: | Cancer Biology Training Grant 5T32CA9302-35 | ||||
| Quality control: | All antibodies in this study were rigorously titrated. Radiation dose and recovery time was titrated. This experiment was repeated several times, producing consistent results. | ||||
Experiment variables
| Conditions | |
|---|---|
| · 0Gy | Sample_pH2AX_BRCA1_UT_002_cells.fcs |
| · 10Gy | Sample_pH2AX_BRCA1_IR_001_cells.fcs |
| Individuals | |
|---|---|
| · pH2AX_BRCA1 | Sample_pH2AX_BRCA1_IR_001_cells.fcs · Sample_pH2AX_BRCA1_UT_002_cells.fcs |
