Experiment Overview
| Repository ID: | FR-FCM-ZZVD | Experiment name: | Fig 3-4. Application of Vybr-FACS for the identification of the inhibitory effect of ALLN on B. divergens asynchronous cultures and on their the structural variations | MIFlowCyt score: | 80.17% |
| Primary researcher: | Jeny Cursino-Santos | PI/manager: | Cheryl Lobo | Uploaded by: | Jeny Cursino-Santos |
| Experiment dates: | 2016-09-17 - 2016-09-19 | Dataset uploaded: | Oct 2016 | Last updated: | Feb 2017 |
| Keywords: | [Babesia] [FACSDiva] [Vybrant®DyeCycle Green dye] [ALLN] | Manuscripts: | [28207983] |
|
|
| Organizations: |
New York Blood Center, Blood Borne Parasites, New York, New York (USA)
|
||||
| Purpose: | Fig 3-4. Application of Vybr-FACS for the identification of the inhibitory effect of ALLN on B. divergens asynchronous cultures and on their the structural variations . Manuscripted submitted to Cytometry, Part A; "A Novel Flow Cytometric Application Discriminates Among the Effects of Chemical Inhibitors on Various Phases of Babesia divergens Intra-Erythrocytic Cycle". | ||||
| Conclusion: | Under ALLN pressure (ALLN 12-500 µM), -1N and >4N-IRBC sub populations were the most affected by the presence of the drug | ||||
| Comments: | None | ||||
| Funding: | NIH | ||||
| Quality control: | QC of the instrument was performed using the CS&T beads for Diva8 manufactured by BD. Experiment QC: Single stained controls, unstained cells and replicates in treated and control samples. | ||||
