Experiment Overview
| Repository ID: | FR-FCM-ZZVE | Experiment name: | Figure 5. Application of Vybr-FACS for the detection of B. divergens response to the presence of ALLN. | MIFlowCyt score: | 79.28% |
| Primary researcher: | Jeny Cursino-Santos | PI/manager: | Cheryl Lobo | Uploaded by: | Jeny Cursino-Santos |
| Experiment dates: | 2016-09-19 - 2016-09-19 | Dataset uploaded: | Oct 2016 | Last updated: | Feb 2017 |
| Keywords: | [Babesia] [FACSDiva] [ALLN] [Vybrant DyeCycle Green dye] | Manuscripts: | [28207983] |
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| Organizations: |
New York Blood Center, Blood Borne Parasites, New York, New York (USA)
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| Purpose: | Figure 5. Application of Vybr-FACS for the detection of B. divergens response to the presence of ALLN. Manuscripted submitted to Cytometry, Part A; "A Novel Flow Cytometric Application Discriminates Among the Effects of Chemical Inhibitors on Various Phases of Babesia divergens Intra-Erythrocytic Cycle". | ||||
| Conclusion: | At the 48h time point (where the drug effect was more prominent) iRBCs harboring multiple parasites were the most affected subpopulation by ALLN, even when the drug was used at low concentration (12-50 µM). | ||||
| Comments: | None | ||||
| Funding: | NIH | ||||
| Quality control: | QC of the instrument was performed using the CS&T beads for Diva8 manufactured by BD. Experiment QC: Single stained controls, unstained cells and replicates in treated and control samples. | ||||
