Experiment Overview
| Repository ID: | FR-FCM-ZZVL | Experiment name: | Figure 4B: Expression of CD45 isoforms. | MIFlowCyt score: | 25.50% |
| Primary researcher: | André Veillette | PI/manager: | Dominique Davidson | Uploaded by: | Dominique Davidson |
| Experiment dates: | 2015-11-20 - | Dataset uploaded: | Nov 2016 | Last updated: | Dec 2016 |
| Keywords: | [CD45 isoforms] [PAG KO mice] | Manuscripts: | |||
| Organizations: |
IRCM, Molecular Oncology, Montreal, Quebec (Canada)
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| Purpose: | Assessment of CD45 expression using antibodies recognizing all CD45 isoforms, CD45RA or CD45RO, in ex vivo or previously-activated wild-type and PAG knock-out CD4+ T cells. | ||||
| Conclusion: | No significant differences in levels of expression of CD45 between wild-type and PAG knock-out CD4+ T cells under all the experimental conditions | ||||
| Comments: | Reference: Davidson et al., 2016, Cell Reports 17, 2776-2788. In the experimental files: CTRL or 4802 = CD4+ T cells from wild-type mice, KO or 4800 or 6014 = CD4+ T cells from PAG KO mice. Data was acquired with a BD FACSCalibur | ||||
| Funding: | Not disclosed | ||||
| Quality control: | None | ||||
