Experiment Overview
| Repository ID: | FR-FCM-ZZVZ | Experiment name: | Fig2.Performance of Vybr-FACS in comparison with other standard methods for parasites detection. | MIFlowCyt score: | 81.35% |
| Primary researcher: | Jeny Cursino-Santos | PI/manager: | Cheryl Lobo | Uploaded by: | Jeny Cursino-Santos |
| Experiment dates: | 2016-01-14 - 2016-01-14 | Dataset uploaded: | Oct 2016 | Last updated: | Feb 2017 |
| Keywords: | [SYBR Green I] [Babesia] [Vybrant®DyeCycleTMGreen dye] [FACSDiva] | Manuscripts: | [28207983] |
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| Organizations: |
New York Blood Center, Blood Borne Parasites, New York, New York (USA)
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| Purpose: | Manuscripted submitted to Cytometry, Part A; "A Novel Flow Cytometric Application Discriminates Among the Effects of Chemical Inhibitors on Various Phases of Babesia divergens Intra-Erythrocytic Cycle". Fig. 2 The performance of Vybr-FACS against SYBR-FACS and light microscopy was assessed with the same set of serially diluted (SD) samples. | ||||
| Conclusion: | Vybr-FACS yields parasitemia measurements comparable to other standard methods. Vybr-FACS provides a more accurate measurement of the intracellular parasite load when compared to SYBR-FACS | ||||
| Comments: | None | ||||
| Funding: | NIH | ||||
| Quality control: | QC of the instrument was performed using the CS&T beads for Diva8 manufactured by BD. Experiment QC: Single stained controls, unstained cells and replicates in treated and control samples. | ||||
Experiment variables
| Sample Type | |
|---|---|
| · Sybr-Not infected RBC-US.fcs (Not infected RBC-US) | Sybr-Not infected RBC-US.fcs · Sybr_SD1_US.fcs |
