Experiment Overview
| Repository ID: | FR-FCM-ZZWU | Experiment name: | OMIP-030: Characterization of eight major human CD4+ T helper subsets, including Tregs, via surface markers | MIFlowCyt score: | 77.50% |
| Primary researcher: | Gerhard Wingender | PI/manager: | Gerhard Wingender | Uploaded by: | Gerhard Wingender |
| Experiment dates: | 2012-11-01 - 2013-10-31 | Dataset uploaded: | Feb 2015 | Last updated: | Oct 2015 |
| Keywords: | [human] [CD4+ T cells] [PBMCs] [T helper] [T cell subsets] [mNKT/MAIT cells] | Manuscripts: |
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| Organizations: |
La Jolla Institute for Allergy and Immunology, La Jolla, CA (USA)
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| Purpose: | Characterization of eight major human CD4+ T helper subsets, including Tregs, via surface markers | ||||
| Conclusion: | Providing a staining panel that allows the enumerate of the major CD4+ T cells subsets in one stain via surface markers | ||||
| Comments: | None | ||||
| Funding: | NIH grants RO1 AI45053, AI69296 and R37 AI71922 | ||||
| Quality control: | Instrument QC: - All data was acquired on the same flow cytometer using the same cytometer settings. - Cytometer passed QC tests including instrument setup beads and detector testing, prior to acquisition. Sample QC: All biological samples processed, stored, and stained in the same way. Data QC: All Data was analyzed by the same person with uniform application of gating strategy across all samples. | ||||
