Experiment Overview
| Repository ID: | FR-FCM-ZZWX | Experiment name: | Inactivation of urod in zebrafish blood cells | MIFlowCyt score: | 63.75% |
| Primary researcher: | Ellen Durand | PI/manager: | Leonard Zon | Uploaded by: | Ellen Durand |
| Experiment dates: | 2014-10-24 - 2014-10-24 | Dataset uploaded: | Jan 2015 | Last updated: | Feb 2015 |
| Keywords: | [CRISPR] [zebrafish] [urod] | Manuscripts: | |||
| Organizations: |
Boston Children's Hospital, Hematology/Oncology, Boston, MA (United States)
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| Purpose: | We wanted to evaluate the proportion of red blood cells in pcmlc2:GFP, zU6:gRNA urod, gata1:zCas9 embryos that show red fluorescence. | ||||
| Conclusion: | We detect red fluorescent cells in the blood of pcmlc2:GFP, zU6:gRNA urod, gata1:zCas9 zebrafish embryos (36 hpf and 48hpf). | ||||
| Comments: | However, a large percentage of these cells appear to be dead/dying compared to WT siblings, indicating that they are particularly fragile and that we may have an underestimation of the proportion of urod mutant cells at a given timepoint. | ||||
| Funding: | Not disclosed | ||||
| Quality control: | Compared injected embryos to WT sibling controls and carefully controlled for auto-fluorescence during FACS. | ||||
Experiment variables
| Doses | |
|---|---|
| · Un-injected embryos | WT siblings.fcs |
| · Injected embryos | pcmlc2_GFPU6_gRNAurodgata1_Cas9.fcs |
| Timepoints | |
|---|---|
| · 36 hpf | WT siblings.fcs · pcmlc2_GFPU6_gRNAurodgata1_Cas9.fcs |
| Sample Type | |
|---|---|
| · Blood cells | WT siblings.fcs · pcmlc2_GFPU6_gRNAurodgata1_Cas9.fcs |
