Experiment Overview
| Repository ID: | FR-FCM-ZZX2 | Experiment name: | Hematopoietic phenotype changes as a result of loss of Meis1 expression with or without NAC treatment in mouse bone marrow | MIFlowCyt score: | 34.50% |
| Primary researcher: | Michelle Miller | PI/manager: | Michelle Miller | Uploaded by: | Michelle Miller |
| Experiment dates: | 2013-08-01 - 2013-09-01 | Dataset uploaded: | Dec 2015 | Last updated: | Feb 2016 |
| Keywords: | [Population Identification] [hematopoietic stem cells] | Manuscripts: | |||
| Organizations: |
BC Cancer Agency, Terry Fox Lab, Vancouver, BC (Canada)
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| Purpose: | Loss of Meis1 results in changes in frequency of sub populations of hematopoietic cells. This has been postulated to be, at least in part, via reactive oxygen species. We deleted Meis1 in the presence and absence of ROS scavenger N-acetylcysteine and examined the impact on hematopoietic sub-populations. | ||||
| Conclusion: | NAC treatment abolishes the difference in numbers of HSC and MkP progenitors between Meis1 -/+ and Meis1 -/- mice | ||||
| Comments: | None | ||||
| Funding: | Terry Fox Foundation, MSFHR | ||||
| Quality control: | Single stain and FMO controls | ||||
Experiment variables
| Conditions | |
|---|---|
| · Controls | Aug 7 2013_CLP FMO 127_019.fcs · Aug 7 2013_CLP FMO Sca PE_020.fcs · Aug 7 2013_CLP FMO cKit APC_021.fcs · Aug 7 2013_CMP FMO CD1632_014.fcs · Aug 7 2013_CMP FMO CD34_015.fcs · Aug 7 2013_CTL Gr1APCCy7_013.fcs · Aug 7 2013_CTL LinPerCP_008.fcs · Aug 7 2013_CTL Sca1APC_010.fcs · Aug 7 2013_CTL Sca1FITC_012.fcs · Aug 7 2013_CTL Sca1PECy7_011.fcs · Aug 7 2013_CTL Sca1PE_009.fcs · Aug 7 2013_CTL unstained_007.fcs · Aug 7 2013_Erythroid DAPI_001.fcs · Aug 7 2013_Erythroid FITC_003.fcs · Aug 7 2013_Erythroid PE_002.fcs · Aug 8 2013_CTL CD1632 PE_009.fcs · Aug 8 2013_CTL CLP FMO 127_020.fcs · Aug 8 2013_CTL CLP FMO Sca1PE_021.fcs · Aug 8 2013_CTL CLP FMO cKitAPC_022.fcs · Aug 8 2013_CTL CLP PerCP55_019.fcs · Aug 8 2013_CTL CMP FMO 1632_017.fcs · Aug 8 2013_CTL CMP FMO CD34_018.fcs · Aug 8 2013_CTL DAPI only_010.fcs · Aug 8 2013_CTL Gr1 APCCy7_014.fcs · Aug 8 2013_CTL Lin PerCP_011.fcs · Aug 8 2013_CTL Sca APC_013.fcs · Aug 8 2013_CTL Sca PECy7_012.fcs · Aug 8 2013_CTL Sca1 FITC_015.fcs · Aug 8 2013_CTL Sca1 PE_016.fcs · Aug 8 2013_Eryth CD71 PE_004.fcs · Aug 8 2013_Eryth DAPI_002.fcs · Aug 8 2013_Eryth Ter119 FITC_003.fcs · Aug 8 2013_Eryth unstained_001.fcs · Aug 9 2013_CLP FMO CD127_026.fcs · Aug 9 2013_CLP FMO Sca1_025.fcs · Aug 9 2013_CLP FMO cKit_027.fcs · Aug 9 2013_CMP FMO 1632_019.fcs · Aug 9 2013_CMP FMO 34_020.fcs · Aug 9 2013_CTL CD127 PerCP55_013.fcs · Aug 9 2013_CTL CD1632 PE_011.fcs · Aug 9 2013_CTL DAPI_010.fcs · Aug 9 2013_CTL Gr1 APCCy7_018.fcs · Aug 9 2013_CTL Lin PerCP55_014.fcs · Aug 9 2013_CTL ScaAPC_017.fcs · Aug 9 2013_CTL ScaFITC_016.fcs · Aug 9 2013_CTL ScaPECy7_015.fcs · Aug 9 2013_CTL ScaPE_012.fcs · Aug 9 2013_CTL unstained_009.fcs · Aug 9 2013_Eryth DAPI_002.fcs · Aug 9 2013_Eryth PE CD71_003.fcs · Aug 9 2013_Eryth Ter119 FITC_004.fcs · Aug 9 2013_Eryth unstained_001.fcs |
| · Meis1 -/+ PBS | Aug 7 2013_CLP Sample3_024.fcs · Aug 7 2013_CMP Sample 3_018.fcs · Aug 7 2013_Erythroid Sample 3_006.fcs · Aug 7 2013_Lin Sample 3_027.fcs · Aug 8 2013_CLP Sample 1_027.fcs · Aug 8 2013_CMP Sample 1_023.fcs · Aug 8 2013_Eryth Sample 1_005.fcs · Aug 8 2013_Lin Sample 1_031.fcs · Aug 9 2013_CLP Sample 2_029.fcs · Aug 9 2013_CMP Sample 2_022.fcs · Aug 9 2013_Eryth Sample 2_006.fcs · Aug 9 2013_Lin Sample 2_033.fcs |
| · Meis1 -/+ NAC | Aug 7 2013_CLP Sample2_023.fcs · Aug 7 2013_CMP Sample 2_017.fcs · Aug 7 2013_Erythroid Sample 2_005.fcs · Aug 7 2013_Lin Sample 2_026.fcs · Aug 8 2013_CLP Sample 3_029.fcs · Aug 8 2013_CMP Sample 3_025.fcs · Aug 8 2013_Eryth Sample 3_007.fcs · Aug 8 2013_Lin Sample 3_033.fcs · Aug 9 2013_CLP Sample 4_031.fcs · Aug 9 2013_CMP Sample 4_024.fcs · Aug 9 2013_Eryth Sample 4_008.fcs · Aug 9 2013_Lin Sample 4_035.fcs |
| · Meis1 -/- PBS | Aug 7 2013_CLP Sample1_022.fcs · Aug 7 2013_CMP Sample 1_016.fcs · Aug 7 2013_Erythroid Sample 1_004.fcs · Aug 7 2013_Lin Sample 1_025.fcs · Aug 8 2013_CLP Sample 2_028.fcs · Aug 8 2013_CMP Sample 2_024.fcs · Aug 8 2013_Eryth Sample 2_006.fcs · Aug 8 2013_Lin Sample 2_032.fcs · Aug 9 2013_CLP Sample 3_030.fcs · Aug 9 2013_CMP Sample 3_023.fcs · Aug 9 2013_Eryth Sample 3_007.fcs · Aug 9 2013_Lin Sample 3_034.fcs |
| · Meis1 -/- NAC | Aug 8 2013_CLP Sample 4_030.fcs · Aug 8 2013_CMP Sample 4_026.fcs · Aug 8 2013_Eryth Sample 4_008.fcs · Aug 8 2013_Lin Sample 4_034.fcs · Aug 9 2013_CLP Sample 1_028.fcs · Aug 9 2013_CMP Sample 1_021.fcs · Aug 9 2013_Eryth Sample 1_005.fcs · Aug 9 2013_Lin Sample 1_032.fcs |
| Sample Type | |
|---|---|
| · Controls | Aug 7 2013_CTL Gr1APCCy7_013.fcs · Aug 7 2013_CTL LinPerCP_008.fcs · Aug 7 2013_CTL Sca1APC_010.fcs · Aug 7 2013_CTL Sca1FITC_012.fcs · Aug 7 2013_CTL Sca1PECy7_011.fcs · Aug 7 2013_CTL Sca1PE_009.fcs · Aug 7 2013_CTL unstained_007.fcs · Aug 8 2013_CTL CD1632 PE_009.fcs · Aug 8 2013_CTL DAPI only_010.fcs · Aug 8 2013_CTL Gr1 APCCy7_014.fcs · Aug 8 2013_CTL Sca APC_013.fcs · Aug 8 2013_CTL Sca PECy7_012.fcs · Aug 8 2013_CTL Sca1 FITC_015.fcs · Aug 8 2013_CTL Sca1 PE_016.fcs · Aug 9 2013_CTL CD127 PerCP55_013.fcs · Aug 9 2013_CTL CD1632 PE_011.fcs · Aug 9 2013_CTL DAPI_010.fcs · Aug 9 2013_CTL Gr1 APCCy7_018.fcs · Aug 9 2013_CTL ScaAPC_017.fcs · Aug 9 2013_CTL ScaFITC_016.fcs · Aug 9 2013_CTL ScaPECy7_015.fcs · Aug 9 2013_CTL ScaPE_012.fcs · Aug 9 2013_CTL unstained_009.fcs |
| · CMP | Aug 7 2013_CMP FMO CD1632_014.fcs · Aug 7 2013_CMP FMO CD34_015.fcs · Aug 7 2013_CMP Sample 1_016.fcs · Aug 7 2013_CMP Sample 2_017.fcs · Aug 7 2013_CMP Sample 3_018.fcs · Aug 8 2013_CMP Sample 1_023.fcs · Aug 8 2013_CMP Sample 2_024.fcs · Aug 8 2013_CMP Sample 3_025.fcs · Aug 8 2013_CMP Sample 4_026.fcs · Aug 8 2013_CTL CMP FMO 1632_017.fcs · Aug 8 2013_CTL CMP FMO CD34_018.fcs · Aug 9 2013_CMP FMO 1632_019.fcs · Aug 9 2013_CMP FMO 34_020.fcs · Aug 9 2013_CMP Sample 1_021.fcs · Aug 9 2013_CMP Sample 2_022.fcs · Aug 9 2013_CMP Sample 3_023.fcs · Aug 9 2013_CMP Sample 4_024.fcs |
| · CLP | Aug 7 2013_CLP FMO 127_019.fcs · Aug 7 2013_CLP FMO Sca PE_020.fcs · Aug 7 2013_CLP FMO cKit APC_021.fcs · Aug 7 2013_CLP Sample1_022.fcs · Aug 7 2013_CLP Sample2_023.fcs · Aug 7 2013_CLP Sample3_024.fcs · Aug 8 2013_CLP Sample 1_027.fcs · Aug 8 2013_CLP Sample 2_028.fcs · Aug 8 2013_CLP Sample 3_029.fcs · Aug 8 2013_CLP Sample 4_030.fcs · Aug 8 2013_CTL CLP FMO 127_020.fcs · Aug 8 2013_CTL CLP FMO Sca1PE_021.fcs · Aug 8 2013_CTL CLP FMO cKitAPC_022.fcs · Aug 8 2013_CTL CLP PerCP55_019.fcs · Aug 9 2013_CLP FMO CD127_026.fcs · Aug 9 2013_CLP FMO Sca1_025.fcs · Aug 9 2013_CLP FMO cKit_027.fcs · Aug 9 2013_CLP Sample 1_028.fcs · Aug 9 2013_CLP Sample 2_029.fcs · Aug 9 2013_CLP Sample 3_030.fcs · Aug 9 2013_CLP Sample 4_031.fcs |
| · Eryth | Aug 7 2013_Erythroid DAPI_001.fcs · Aug 7 2013_Erythroid FITC_003.fcs · Aug 7 2013_Erythroid PE_002.fcs · Aug 7 2013_Erythroid Sample 1_004.fcs · Aug 7 2013_Erythroid Sample 2_005.fcs · Aug 7 2013_Erythroid Sample 3_006.fcs · Aug 8 2013_Eryth CD71 PE_004.fcs · Aug 8 2013_Eryth DAPI_002.fcs · Aug 8 2013_Eryth Sample 1_005.fcs · Aug 8 2013_Eryth Sample 2_006.fcs · Aug 8 2013_Eryth Sample 3_007.fcs · Aug 8 2013_Eryth Sample 4_008.fcs · Aug 8 2013_Eryth Ter119 FITC_003.fcs · Aug 8 2013_Eryth unstained_001.fcs · Aug 9 2013_Eryth DAPI_002.fcs · Aug 9 2013_Eryth PE CD71_003.fcs · Aug 9 2013_Eryth Sample 1_005.fcs · Aug 9 2013_Eryth Sample 2_006.fcs · Aug 9 2013_Eryth Sample 3_007.fcs · Aug 9 2013_Eryth Sample 4_008.fcs · Aug 9 2013_Eryth Ter119 FITC_004.fcs · Aug 9 2013_Eryth unstained_001.fcs |
| · Lin | Aug 7 2013_Lin Sample 1_025.fcs · Aug 7 2013_Lin Sample 2_026.fcs · Aug 7 2013_Lin Sample 3_027.fcs · Aug 8 2013_CTL Lin PerCP_011.fcs · Aug 8 2013_Lin Sample 1_031.fcs · Aug 8 2013_Lin Sample 2_032.fcs · Aug 8 2013_Lin Sample 3_033.fcs · Aug 8 2013_Lin Sample 4_034.fcs · Aug 9 2013_CTL Lin PerCP55_014.fcs · Aug 9 2013_Lin Sample 1_032.fcs · Aug 9 2013_Lin Sample 2_033.fcs · Aug 9 2013_Lin Sample 3_034.fcs · Aug 9 2013_Lin Sample 4_035.fcs |
