Experiment Overview
| Repository ID: | FR-FCM-ZZX6 | Experiment name: | Profiling of Polarized Monocytes I | MIFlowCyt score: | 76.19% |
| Primary researcher: | Yann Abraham | PI/manager: | Yann Abraham | Uploaded by: | Yann Abraham |
| Experiment dates: | 2015-12-17 - 2016-03-18 | Dataset uploaded: | Dec 2015 | Last updated: | May 2017 |
| Keywords: | [monocytes] [macrophage polarization] [dexamethasone] | Manuscripts: | [27933748] [27933748] |
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| Organizations: |
Novartis Pharma AG, NIBR / DMP, Basel, (Switzerland)
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| Purpose: | The goal of this experiment is to compare the polarization induced by two experimental compounds at 10uM with that induced by Dexamethasone. | ||||
| Conclusion: | One of the compound has a similar effect to Dexamethasone, while the other one induces a different response | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | Instrument performance was monitored using labelled beads mixed with cells (1/10). Dead cells and debris were manually gated. | ||||
Experiment variables
| Doses | |
|---|---|
| · NA | cy150124_1_Mono_395_MCSF_cells_found.fcs · cy150126_2_Mono_395_Dex_cells_found.fcs · cy150131_2_Mono_395_Dex_cells_found.fcs · cy150133_5_Mono_396_MCSF_cells_found.fcs · cy150134_6_Mono_396_Dex_cells_found.fcs · cy150137_9_Mono_398_MCSF_cells_found.fcs · cy150138_10_Mono_398_Dex_cells_found.fcs · cy150141_13_Mono_801_MCSF_cells_found.fcs · cy150142_14_Mono_801_Dex_cells_found.fcs · cy150145_17_Mono_802_MCSF_cells_found.fcs · cy150146_18_Mono_802_Dex_cells_found.fcs |
| · 10uM | cy150127_3_Mono_395_cpd6_cells_found.fcs · cy150130_4_Mono_395_cpd11_cells_found.fcs · cy150132_3_Mono_395_cpd6_cells_found.fcs · cy150135_7_Mono_396_cpd6_cells_found.fcs · cy150136_8_Mono_396_cpd11_cells_found.fcs · cy150139_11_Mono_398_cpd6_cells_found.fcs · cy150140_12_Mono_398_cpd11_cells_found.fcs · cy150143_15_Mono_801_cpd6_cells_found.fcs · cy150144_16_Mono_801_cpd11_cells_found.fcs · cy150147_19_Mono_802_cpd6_cells_found.fcs · cy150148_20_Mono_802_cpd11_cells_found.fcs · cy150149_7_Mono_396_cpd6_cells_found.fcs |
| Conditions | |
|---|---|
| · M-CSF | cy150124_1_Mono_395_MCSF_cells_found.fcs · cy150133_5_Mono_396_MCSF_cells_found.fcs · cy150137_9_Mono_398_MCSF_cells_found.fcs · cy150141_13_Mono_801_MCSF_cells_found.fcs · cy150145_17_Mono_802_MCSF_cells_found.fcs |
| · Dex | cy150126_2_Mono_395_Dex_cells_found.fcs · cy150131_2_Mono_395_Dex_cells_found.fcs · cy150134_6_Mono_396_Dex_cells_found.fcs · cy150138_10_Mono_398_Dex_cells_found.fcs · cy150142_14_Mono_801_Dex_cells_found.fcs · cy150146_18_Mono_802_Dex_cells_found.fcs |
| · Cpd6 | cy150127_3_Mono_395_cpd6_cells_found.fcs · cy150132_3_Mono_395_cpd6_cells_found.fcs · cy150135_7_Mono_396_cpd6_cells_found.fcs · cy150139_11_Mono_398_cpd6_cells_found.fcs · cy150143_15_Mono_801_cpd6_cells_found.fcs · cy150147_19_Mono_802_cpd6_cells_found.fcs · cy150149_7_Mono_396_cpd6_cells_found.fcs |
| · Cpd11 | cy150130_4_Mono_395_cpd11_cells_found.fcs · cy150136_8_Mono_396_cpd11_cells_found.fcs · cy150140_12_Mono_398_cpd11_cells_found.fcs · cy150144_16_Mono_801_cpd11_cells_found.fcs · cy150148_20_Mono_802_cpd11_cells_found.fcs |
| Individuals | |
|---|---|
| · 395 | cy150124_1_Mono_395_MCSF_cells_found.fcs · cy150126_2_Mono_395_Dex_cells_found.fcs · cy150127_3_Mono_395_cpd6_cells_found.fcs · cy150130_4_Mono_395_cpd11_cells_found.fcs · cy150131_2_Mono_395_Dex_cells_found.fcs · cy150132_3_Mono_395_cpd6_cells_found.fcs |
| · 396 | cy150133_5_Mono_396_MCSF_cells_found.fcs · cy150134_6_Mono_396_Dex_cells_found.fcs · cy150135_7_Mono_396_cpd6_cells_found.fcs · cy150136_8_Mono_396_cpd11_cells_found.fcs · cy150149_7_Mono_396_cpd6_cells_found.fcs |
| · 398 | cy150137_9_Mono_398_MCSF_cells_found.fcs · cy150138_10_Mono_398_Dex_cells_found.fcs · cy150139_11_Mono_398_cpd6_cells_found.fcs · cy150140_12_Mono_398_cpd11_cells_found.fcs |
| · 801 | cy150141_13_Mono_801_MCSF_cells_found.fcs · cy150142_14_Mono_801_Dex_cells_found.fcs · cy150143_15_Mono_801_cpd6_cells_found.fcs · cy150144_16_Mono_801_cpd11_cells_found.fcs |
| · 802 | cy150145_17_Mono_802_MCSF_cells_found.fcs · cy150146_18_Mono_802_Dex_cells_found.fcs · cy150147_19_Mono_802_cpd6_cells_found.fcs · cy150148_20_Mono_802_cpd11_cells_found.fcs |
